| 橡胶树叶片染色体制片方法的优化 |
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| 引用本文： | 高和琼,王英,金鸽,邱海燕,庄南生.橡胶树叶片染色体制片方法的优化[J].热带作物学报,2009,30(5):565-569. |
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| 作者姓名： | 高和琼 王英 金鸽 邱海燕 庄南生 |
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| 作者单位： | 1. 中国热带农业科学院橡胶研究所,海南儋州,571737;海南大学儋州校区农学院,海南儋州,571737
2. 海南大学儋州校区农学院,海南儋州,571737 |
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| 基金项目： | 海南省自然科学基金指导性项目 |
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| 摘 要： | 采用压片法、酶解去壁低渗法和悬液法等方法,对橡胶树热研7-33-97古铜期嫩叶染色体制片及其过程中预处理药剂、预处理时间、前低渗温度、酶解时间等因子进行了探讨.结果表明:悬液法更适合橡胶树叶片染色体制片.最佳预处理药剂为0.1%的秋水仙素和0.002mol/L 8-羟基喹啉混合液,预处理时间为2 h.酶解温度37℃,时间为5～7 h.
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| 关 键 词： | 橡胶树 热研7-33-97 染色体制片 优化 |
Optimization of Chromosome Preparations of Hevea Leaves |
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| Gao Heqiong,Wang Ying,Jin Ge,Qiu Haiyan and Zhuang Nansheng.Optimization of Chromosome Preparations of Hevea Leaves[J].Chinese Journal of Tropical Crops,2009,30(5):565-569. |
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| Authors: | Gao Heqiong Wang Ying Jin Ge Qiu Haiyan and Zhuang Nansheng |
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| Institution: | Gao Heqiong1,2,Wang Ying2,Jin Ge2,Qiu Haiyan2,Zhuang Nansheng2 1 Rubber Research Insititute,CATAS,Danzhou,Hainan 571737 2 College of Agronomy,Hainan University,Hainan 571737 |
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| Abstract: | Chromosome preparation is of great significance for chromosome and karyotype analysis in ploidy breeding, interspecific hybridization and germplasm analysis of Hevea brasiliensis Muell. Arg. Chromosome preparations of bronze-coloured tender leaves of Reyan 7-33-97 were made by using the squash method, the enzyme and low osmotic method and suspension method, and some important factors during the preparation, such as the chemicals for pretreatment, pretreatment time, early low osmosis temperature, enzymolysis... |
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| Keywords: | Hevea brasiliensis Reyan7-33-97 chromosome preparation optimization |
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