| 不同基因型烤烟苗期氮代谢对相关酶活性的影响 |
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| 引用本文： | 李中民,杨铁钊,段旺军,李亚培.不同基因型烤烟苗期氮代谢对相关酶活性的影响[J].浙江农业学报,2011,23(3). |
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| 作者姓名： | 李中民 杨铁钊 段旺军 李亚培 |
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| 作者单位： | 河南农业大学,烟草学院,河南郑州450002 |
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| 基金项目： | 河南省烟草专卖局科技攻关项目 |
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| 摘 要： | 采用沙培方法,选用不同基因型烤烟‘K326'、‘中烟90'和‘净叶黄',研究氮形态对不同烤烟幼苗叶部、根部的酶活性及可溶性蛋白含量的影响.研究发现,在NO3 -N或NH4+ -N不同处理水平下,‘净叶黄'叶部硝酸还原酶(NR)的活性较高,‘中烟90'根部NR的活性较高,在NH4+ -N的处理水平下NR的活性高于NO3- -N处理水平;‘K326'叶部和根部的谷氨酰胺合成酶(GS)的活力较高,根部的GS活力远大于叶部,并且在NH4+ -N的处理水平下叶部的GS活性低于NO3- -N处理水平,根部NH4+ -N处理水平下GS活性高于的NO3- -N处理水平;可溶性蛋白的含量具体表现为‘净叶黄'叶部较高,‘中烟90'根部较高.结果表明不同基因型烤烟间根部、叶部NR和GS活性以及可溶性蛋白含量与氮素形态有关,并且与氮素水平不呈正相关.
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| 关 键 词： | 烤烟 氮素形态 谷氨酰胺合成酶 可溶性蛋白 |
Effects of different genotypes of flue-cured tobacco on nitrogen metabolism-related enzyme activity |
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| LI Zhong-min,YANG Tie-zhao,DUAN Wang-jun,LI Ya-pei.Effects of different genotypes of flue-cured tobacco on nitrogen metabolism-related enzyme activity[J].Acta Agriculturae Zhejiangensis,2011,23(3). |
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| Authors: | LI Zhong-min YANG Tie-zhao DUAN Wang-jun LI Ya-pei |
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| Institution: | LI Zhong-min,YANG Tie-zhao,DUAN Wang-jun,LI Ya-pei(College of Tobacco,Henan Agricultural University,Zhengzhou 450002,China) |
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| Abstract: | The effects of nitrogen forms on different flue-cured tobacco seedling roots,leaf soluble protein and associated enzyme activities were studied in different genotypes of flue-cured tobacco K326,Zhongyan 90(ZY90) and Jingyehuang(JYH).The results showed that in different NO-3-N and NH4 +-N treatments,nitrate reductase(NR) activity in JYH leaves was higher than the other two genotypes,and NR activity in ZY90 roots was higher,furthermore,NR activity in NH4 +-N treatments was higher than in NO-3-N treatments.Glu... |
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| Keywords: | flue-cured tobacco nitrate reductase glutamine synthetase soluble protein |
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