| 用于低乳糖牛奶生产的乳糖酶添加剂候选菌株筛选及粗酶性质研究 |
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| 引用本文： | 李荷,张博,张敏文,顾取良.用于低乳糖牛奶生产的乳糖酶添加剂候选菌株筛选及粗酶性质研究[J].河南农业科学,2011,40(4):138-141,145. |
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| 作者姓名： | 李荷 张博 张敏文 顾取良 |
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| 作者单位： | 广东药学院生物化学与分子生物学系,广东广州,510006 |
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| 基金项目： | 国家自然科学基金项目，广东省科技计划项目，广东省自然科学基金博士启动基金，广东药学院博士启动项目 |
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| 摘 要： | 在低乳糖牛奶加工过程中需要高活性的低温乳糖酶作为添加剂。为此,从采集土样中分离纯化获得1株低乳糖奶加工过程中需要的高乳糖酶活性候选菌株,应用形态学和分子生物学方法,初步鉴定该菌株为动球菌属(Planococcus sp.),命名为Planococcus sp.Y。研究表明,该菌株是1株低温菌株,其最适生长温度为25℃,最佳生长pH值为7.0,所产酶为胞内酶,粗酶液的最适酸碱度和温度分别为pH7.0和25℃。
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| 关 键 词： | 乳糖酶 筛选 低温菌 |
Screening of A Psychrotolerant Bacterium of Planococcus sp.Y Producing Low Temperature Lactose and Preliminary Study on Its Enzyme Activity |
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| LI He,ZHANG Bo,ZHANG Min-wen,GU Qu-liang.Screening of A Psychrotolerant Bacterium of Planococcus sp.Y Producing Low Temperature Lactose and Preliminary Study on Its Enzyme Activity[J].Journal of Henan Agricultural Sciences,2011,40(4):138-141,145. |
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| Authors: | LI He ZHANG Bo ZHANG Min-wen GU Qu-liang |
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| Institution: | (Department of Biochemistry&Molecular Biology,Guangdong Pharmaceutical University,Guangzhou 510006,China) |
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| Abstract: | High activity of cold-adapted low lactase as an additive is necessary in processing low lactose milk.The psychrotrophic and bacterium strain was isolated from surficial soils.A strain capable of producing psychrotrophic a lactase was identified as a Planococcus sp.Y according to the morphological and molecular characteristics.The optimal conditions for the growth of the strain were examined.The optimal temperature and pH value for growth were 25℃ and 7.0,respectively.Study on the activity of the lactase showed that the optimal temperature and pH for the activity of the crude enzyme was 25℃ and 7.0,respectively.The lactase from Planococcus sp.Y was located intracellularly.This will provide a foundation for producing low lactose milk in future. |
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| Keywords: | Lactase Screening Psychrotolerant bacterium |
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