| 春兰GLO基因的克隆和实时定量表达分析 |
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| 引用本文： | 向林,李伯钧,秦德辉,郭方其,吴超,孙崇波.春兰GLO基因的克隆和实时定量表达分析[J].浙江农业学报,2011,23(3). |
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| 作者姓名： | 向林 李伯钧 秦德辉 郭方其 吴超 孙崇波 |
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| 作者单位： | 浙江省农业科学院园艺研究所,浙江杭州,310021 |
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| 基金项目： | 浙江省自然科学基金项目，浙江省科技计划项目，浙江省花卉产业科技创新团队项目 |
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| 摘 要： | 采用RT-PCR结合RACE技术从春兰(Cymbidium goeringii)中分离到一个GLO基因.该基因含有一个630 bp的开放阅读框(ORF),共编码210个氨基酸.系统进化树分析显示,该基因属于B类MADS-box基因的PI/GLO家族,其编码的蛋白与其他植物PI/GLO类蛋白具有很高的同源性,命名为CgGLO(登录号HM106984).实时荧光定量表达分析表明,CgGLO主要在第二轮花器官唇瓣和花瓣中表达,在萼片、子房和叶片中表达较少,在蕊柱和根中表达量最少,这种表达模式支持了van Tunen对ABC模型的修正,也显示了CgGLO基因可能在春兰花器官以及子房的形成过程中起着重要作用.
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| 关 键 词： | 春兰 ABC模型 GLO基因 实时荧光定量表达 |
Cloning and Real-time Expression Analysis of GLO Gene from Cymbidium goeringii |
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| XIANG Lin,LI Bo-jun,QIN De-hui,GUO Fang-qi,WU Chao,SUN Chong-bo.Cloning and Real-time Expression Analysis of GLO Gene from Cymbidium goeringii[J].Acta Agriculturae Zhejiangensis,2011,23(3). |
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| Authors: | XIANG Lin LI Bo-jun QIN De-hui GUO Fang-qi WU Chao SUN Chong-bo |
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| Institution: | XIANG Lin,LI Bo-jun,QIN De-hui,GUO Fang-qi,WU Chao,SUN Chong-bo(Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China) |
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| Abstract: | In this study,a novel GLO gene was isolated from Cymbidium goeringii by RT-PCR and RACE-PCR techniques.Sequence analysis showed the gene contained an open reading frame of 630 bp encoding a putative protein of 210 amino acids.Homology analysis showed that the deduced protein showed high identity with other PI/GLO proteins from different species,named CgGLO(GenBank accession number HM106984).Phylogenetic tree analysis also indicated that the B-functional gene CgGLO belongs to PI/GLO clade of MADS-box gene fa... |
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| Keywords: | Cymbidium goeringii ABC model GLO gene Real-time quantitative PCR |
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