| DNA在黑土胶体微界面的吸附与解吸特性 |
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| 引用本文： | 王志刚,陈文晶,胡影,徐伟慧,王春龙,吕智航,刘泽平.DNA在黑土胶体微界面的吸附与解吸特性[J].农业环境科学学报,2017,36(10):2058-2062. |
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| 作者姓名： | 王志刚 陈文晶 胡影 徐伟慧 王春龙 吕智航 刘泽平 |
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| 作者单位： | 齐齐哈尔大学生命科学与农林学院, 黑龙江 齐齐哈尔 161006,齐齐哈尔大学生命科学与农林学院, 黑龙江 齐齐哈尔 161006,齐齐哈尔大学生命科学与农林学院, 黑龙江 齐齐哈尔 161006,齐齐哈尔大学生命科学与农林学院, 黑龙江 齐齐哈尔 161006,齐齐哈尔大学生命科学与农林学院, 黑龙江 齐齐哈尔 161006,齐齐哈尔大学生命科学与农林学院, 黑龙江 齐齐哈尔 161006,齐齐哈尔大学生命科学与农林学院, 黑龙江 齐齐哈尔 161006 |
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| 基金项目： | 国家自然科学基金项目（31670375）；齐齐哈尔大学研究生创新项目（YJSCX2015-ZD02） |
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| 摘 要： | 为了揭示DNA在黑土微生物的遗传进化和基因转移机制方面的重要意义,采用平衡法研究了含有机质黑土胶体(Coarse organic colloid,COC)和去有机质黑土胶体(Coarse inorganic colloid,CIC)对DNA的吸附与解吸特性。结果表明:CIC在快速吸附期吸附DNA的速率和数量都小于COC,且更快达到吸附平衡;随着pH值升高,黑土胶体表面吸附DNA数量逐渐减少;Langmuir吸附方程可较好地描述COC和CIC对DNA的等温吸附,COC和CIC的决定系数(R~2)分别为0.972和0.991,且COC的吸附平衡常数(K)大于CIC,说明COC对DNA的吸附亲和能力大于CIC;CIC和COC表面DNA的解吸率分别为63.5%和47.4%。研究结果证明,有机质在黑土胶体吸附DNA的数量和亲和力方面贡献较大,pH是影响黑土胶体吸附DNA的重要因子。
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| 关 键 词： | 黑土胶体 DNA 含有机质胶体 去有机质胶体 等温吸附 解吸 |
| 收稿时间： | 2017/4/27 0:00:00 |
Characteristics of DNA adsorption and desorption on micro-interfaces of black soil colloids |
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| WANG Zhi-gang,CHEN Wen-jing,HU Ying,XU Wei-hui,WANG Chun-long,L&#; Zhi-hang and LIU Ze-ping.Characteristics of DNA adsorption and desorption on micro-interfaces of black soil colloids[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2017,36(10):2058-2062. |
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| Authors: | WANG Zhi-gang CHEN Wen-jing HU Ying XU Wei-hui WANG Chun-long L&#; Zhi-hang and LIU Ze-ping |
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| Institution: | Institute of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China,Institute of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China,Institute of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China,Institute of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China,Institute of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China,Institute of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China and Institute of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China |
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| Abstract: | The characteristics of DNA adsorption and desorption affect the presence of intact DNA and are of great significance for genetic transformation on micro-interfaces of soil colloids. The adsorption and desorption characteristics of DNA by coarse organic colloid(COC) and coarse inorganic colloid(CIC) were studied using the balanced reaction method. The results showed that the rate and quantity of DNA adsorption were lesser on CIC than on COC. The quantity of the adsorbed DNA on black soil colloids decreased gradually with increasing pH. Langmuir adsorption equation could describe the isothermal adsorption of DNA by COC and CIC well, with corresponding determination coefficients(R2) of 0.972 and 0.991, respectively. The equilibrium constant of Langmuir(K) of COC was higher than that of CIC, which proved that the adsorption affinity of COC was better than that of CIC. The rates of DNA desorption from COC and CIC were 63.5% and 47.4%, respectively. The present study indicates that DNA can be adsorbed onto the surface of black colloid, the organic matter contributed greatly to the capability of the DNA adsorption, and that pH is an important factor affecting the adsorption of DNA on black soil colloids. |
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| Keywords: | black soil colloid DNA coarse organic colloid coarse inorganic colloid adsorption isotherms desorption |
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