| 猪圆环病毒2型抗体胶体金检测试纸条的研制 |
| |
| 引用本文： | 王贵华,赵亚荣.猪圆环病毒2型抗体胶体金检测试纸条的研制[J].中国兽药杂志,2012,46(7):41-43. |
| |
| 作者姓名： | 王贵华 赵亚荣 |
| |
| 作者单位： | 北京大北农动物医学研究中心,北京,100097 |
| |
| 摘 要： | 应用胶体金免疫层析技术,采用柠檬酸三钠还原法制备胶体金颗粒,在玻璃纤维膜上包被胶体金标记PCV-2抗原(cap蛋白),在硝酸纤维素膜上检测线和对照线处分别包被PCV-2抗原(cap蛋白)和PCV-2单抗,制成猪圆环病毒2型抗体免疫金标检测试纸条。该试纸条与猪圆环2型抗体酶联免疫检测试剂盒对比,符合率为94.7%,整个实验过程只需15 min,与ELISA试剂盒比较,具有操作方便、快速、直观的优点。
|
| 关 键 词： | 猪圆环病毒2型 抗体 胶体金试纸条 |
| 收稿时间： | 3/16/2012 8:34:27 PM |
| 修稿时间： | 4/1/2012 12:00:00 AM |
Study of Colloidal Gold Strip in Detecting the Ant ibody of Porcine Circovirus Type 2 |
| |
| WANG Gui-hua,WANG Mei-jun,TANG-Bo,CHEN Yi-feng,ZHAO Ya-rong.Study of Colloidal Gold Strip in Detecting the Ant ibody of Porcine Circovirus Type 2[J].Chinese Journal of Veterinary Drug,2012,46(7):41-43. |
| |
| Authors: | WANG Gui-hua WANG Mei-jun TANG-Bo CHEN Yi-feng ZHAO Ya-rong |
| |
| Institution: | Research Center for Veterinary Medicine of Beijing DBN Group, |
| |
| Abstract: | Based on the principle of colloidal gold immunochromatography, with the tri-sodium citrate reduction method, colloidal gold strip was prepared and applied to detect the anti-PCV-2 antibody. PCV-2 and anti-PCV-2 Monoclonal antibody was coated on the test region and control zone respectively. The accuracy of strip is 94.7% compared to ELISA test kit. The whole experimentation merely needed fifteen minutes. This test shows that this strip has the merits of convenient operation, celerity and intuitional. |
| |
| Keywords: | porcine circovirus type 2 antibody immunogold labelling strip |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国兽药杂志》浏览原始摘要信息 |
| 点击此处可从《中国兽药杂志》下载免费的PDF全文 |
|
