| 大豆精加工产品DNA提取方法及转基因检测 |
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| 引用本文： | 栾凤侠,张洪祥,白月.大豆精加工产品DNA提取方法及转基因检测[J].大豆科学,2005,24(3):232-235. |
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| 作者姓名： | 栾凤侠 张洪祥 白月 |
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| 作者单位： | 黑龙江出入境检验检疫局,哈尔滨,150001 |
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| 基金项目： | 国家质量监督检验检疫总局科研项目 |
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| 摘 要： | 针对大豆精加工产品提取DNA纯度和浓度不高的难题,对传统的CTAB法、试剂盒法及改良试剂盒法进行了比较研究,采用改良的试剂盒法快速提取了满足实时荧光定量PCR检测要求的高纯度和高产量DNA,并且利用实时荧光定量PCR定性检测出了大豆精加工产品中的内源基因和外源基因.
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| 关 键 词： | 大豆 精加工产品 DNA提取 |
| 文章编号： | 1000-9841（2005）03-0232-04 |
| 收稿时间： | 2004-11-15 |
| 修稿时间： | 2004-11-15 |
DNA EXTRACTION AND THE GMO DETECTION IN SOYBEAN FINISH MACHINING PRODUCTS |
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| Luan Fengxia,Zhang Hongxiang,Bai Yue.DNA EXTRACTION AND THE GMO DETECTION IN SOYBEAN FINISH MACHINING PRODUCTS[J].Soybean Science,2005,24(3):232-235. |
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| Authors: | Luan Fengxia Zhang Hongxiang Bai Yue |
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| Abstract: | It is difficulty to extract high purify and output DNA of soybean. It was discussed to compare with kit, optimized kit and the traditional CTAB method in this paper. Results showed that the High Pure GMO Sample Preparation Kit was optimized, and the endogenesis and extragenesis in the soya finish machining products by weae datected Realtime--PCR. |
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| Keywords: | Soybean Finish machining products DNA extraction |
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