| 外源物质对小麦幼胚胚性愈伤组织草酸盐氧化酶活性的影响 |
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| 引用本文： | 何盛莲,雷振生,吴政卿,赵献林,杨会民,王美芳,何宁,杨攀,吴剑南.外源物质对小麦幼胚胚性愈伤组织草酸盐氧化酶活性的影响[J].麦类作物学报,2009,29(2). |
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| 作者姓名： | 何盛莲 雷振生 吴政卿 赵献林 杨会民 王美芳 何宁 杨攀 吴剑南 |
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| 作者单位： | 1. 河南省农业科学院小麦研究中心,河南郑州,450002
2. 驻马店市农产品质量检测中心,河南驻马店,463000 |
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| 基金项目： | 河南省杰出科研人才创新工程项目 |
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| 摘 要： | 为了提高小麦幼胚体细胞再生频率,以河南省大面积推广的豫麦49、豫麦18和兰考906为材料,研究了不同浓度外源物质(2,4-D、ABA和AgNO3)对不同基因型小麦幼胚胚性愈伤组织草酸盐氧化酶活性的影响.结果表明,基因型问、不同外源物质浓度间小麦幼胚胚性愈伤组织草酸盐氧化酶活性差异均达到0.01显著水平.基因型间草酸盐氧化酶活性以豫麦18最高、兰考906最低;不同外源物质浓度间草酸盐氧化酶活性以MS培养基上附加2.O～3.0 mg/L 2,4-D、2.0 mg/L 2,4-D+0.1 mg/L ABA和2.0 mg/L 2,4-D+0.1 mg/L ABA+2.5～5.0mg/L AgNO3处理较高.不同外源物质条件下小麦幼胚胚性愈伤组织诱导率与草酸盐氧化酶活性存在极显著相关.
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| 关 键 词： | 小麦 外源物质 胚性愈伤组织 草酸盐氧化酶 |
Effect of Exogenic Substances on Oxalate Oxidase Activities in Embryogenic Calli of Wheat Immature Embryo |
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| HE Sheng-lian,LEI Zhen-sheng,WU Zheng-qing,ZHAO Xian-lin,YANG Hui-min,WANG Mei-fang,HE Ning,YANG Pan,WU Jian-nan.Effect of Exogenic Substances on Oxalate Oxidase Activities in Embryogenic Calli of Wheat Immature Embryo[J].Journal of Triticeae Crops,2009,29(2). |
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| Authors: | HE Sheng-lian LEI Zhen-sheng WU Zheng-qing ZHAO Xian-lin YANG Hui-min WANG Mei-fang HE Ning YANG Pan WU Jian-nan |
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| Institution: | 1.Wheat Research Centre;Henan Academy of Agricultural Sciences;Zhengzhou;Henan 450002;China;2.Agriculture Product Quality Detetion Center of Zhumadian City;Zhumadian;Henan 463000;China |
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| Abstract: | In order to improve the regeneration frequency of somatic embryos of wheat immature embryo,the effects of different concentration exogenic substances on oxalate oxidase activities of immature embryogenic callus were studied in three wheat cultivars(Yumai 18,Yumai 49 and Lankao 906) cultured with more areas in Henan Province.The result indicated that the differences of oxalate oxidase activities of immature embryogenic callus was significant at 0.01 level among different concentrations of exogenic substances... |
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| Keywords: | Wheat Exogenic substances Embryogenic calli Oxalate oxidase |
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