| 响应面法优化泥蚶多糖的提取工艺 |
| |
| 引用本文： | 孔福旺,姜玉新,湛孝东,唐小牛.响应面法优化泥蚶多糖的提取工艺[J].安徽农业科学,2012,40(33):16347-16349. |
| |
| 作者姓名： | 孔福旺 姜玉新 湛孝东 唐小牛 |
| |
| 作者单位： | 皖南医学院寄生虫学教研室,安徽芜湖,241002 |
| |
| 基金项目： | 皖南医学院重点科研项目培育基金 |
| |
| 摘 要： | 目的]利用响应面分析法优化泥蚶多糖的提取工艺。方法]根据中心组合(Box-Benhnken)试验设计原理,以提取温度、提取时间、乙醇浓度3个因素为自变量,多糖得率为响应值,设计了3因素3水平响应面分析试验,以优化泥蚶多糖提取的工艺条件。结果]乙醇浓度是对响应值影响最大的因素,泥蚶多糖提取的最佳工艺条件为提取温度69.6℃,提取时间6.2 h,乙醇浓度78%,在此条件下,泥蚶多糖的提取率为1.635%。结论]研究优化了泥蚶多糖的提取工艺,为大规模制备泥蚶多糖提供参考。
|
| 关 键 词： | 泥蚶 多糖 提取 响应面 |
Optimization of the Extraction Process of Polysaccharides from Tegillarca granosa by Response Surface Methodology (RSM) |
| |
| Institution: | KONG Fu-wang et al(Department of Parasitology,Wannan Medical College,Wuhu,Anhui 241002) |
| |
| Abstract: | Objective] The aim was to use response surface methodology to determine optimum conditions for extraction of polysaccharides from Tegillarca granosa.Method] Response surface methodology with three-factors and three-levels was carried out for optimizing the extraction process of polysaccharides from Tegillarca granosa.A central composite design including independent variables,such as extraction temperature(A),extraction time(B),and ethanol concentration(C) were obtained through Box-Benhnken central combination design.Selected response which evaluates the extraction process was polysaccharide yield.Result] The independent variable with the largest effect on response was ethanol concentration(C).The optimum extraction conditions were found to be extraction temperature 69.6 °C,extraction time 6.2 h,and ethanol concentration of 78%(v/v),respectively.Under these conditions,the extraction efficiency of polysaccharide can increase to 1.635%.Conclusion] Study on the extraction of polysaccharides from Tegillarca granosa could provide a certain theoretical direction for extracting polysaccharides from Tegillarca granosa on a large scale. |
| |
| Keywords: | Tegillarca granosa Polysaccharides Extraction Response Surface Methodology |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
