| 枳早期结瘤素样基因PtBCP1的克隆和分析 |
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| 引用本文： | 姚利晓,马园园,李凤龙,许兰珍,雷天刚,彭爱红,何永睿,陈善春.枳早期结瘤素样基因PtBCP1的克隆和分析[J].安徽农业科学,2012,40(33):16102-16106. |
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| 作者姓名： | 姚利晓 马园园 李凤龙 许兰珍 雷天刚 彭爱红 何永睿 陈善春 |
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| 作者单位： | 中国农业科学院/西南大学柑桔研究所,国家柑桔品种改良中心,国家柑桔工程技术研究中心,重庆400712 |
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| 基金项目： | 长江学者和创新团队发展计划项目，柑桔产业技术体系(CARS-27)农业部公益性行业(农业)科研专项，重庆市自然科学基金计划项目 |
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| 摘 要： | 目的]克隆枳早期结瘤素样基因PtBCP1并对其序列进行分析。方法]以枳消减文库中的C28 EST为种子序列进行电子克隆,据此设计引物进行PCR,以获得枳早期结瘤素样基因PtBCP1的全长cDNA和DNA序列,并对获得的序列进行生物信息学分析。结果]PtBCP1基因由2个外显子和1个内含子组成,在枳幼苗根中的表达量是叶中的140倍,该基因编码的蛋白含131个氨基酸残基,预测分子量为14.0 kD,理论等电点为8.75,具有N端信号肽和PCLD(PFMD:PF02298)功能域,但无完整的铜离子结合位点,属于早期结瘤素样蛋白。结论]为进一步研究PtBCP1基因的生物学功能奠定了基础。
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| 关 键 词： | 枳 早期结瘤素样基因 克隆 |
Clone and Analysis of A Phytocyanin-related Early Nodulin-like gene, PtBCP1 from Poncirus trifoliate |
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| Institution: | YAO Li-xiao et al(Citrus Research Institute,Chinese Academy of Agricultural Sciences/SouthWest University,National Citrus Improvement Center,National Citrus Engineering Research Center,Chongqing 400712) |
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| Abstract: | Objective] The aim was to clone and analyze the sequence of phytocyanin-related early nodulin-like gene(PtBCP1) from Poncirus trifoliata.Method] The electronic cloning was done by using the C28 EST of Poncirus trifoliata subtractive library as seed sequences and the RT-PCR primers were designed by that.Then the PtBCP1 cDNA and DNA of Poncirus trifoliate were cloned by PCR and the sequences were analyzed by bioinformatics methods.Result] The PtBCP1 gene included two exons and one intron,and was 140 times of expression in the seeding root of Poncirus trifoliata than that of in leaves.The gene encoded a polypeptide of 131 amino acids with a 14 kD molecular weight and 8.75 isoelectric point.The PtBCP1 protein was a early nodulin-like protein,with a signal peptide in the N-terminal and a plastocyanin-like domain in the C-terminal,and without the complete copper-binding sites.Conclusion] It prepared the ground for the further research of PtBCP1 gene biological function. |
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| Keywords: | Poncirus trifoliata Early nodulin-like gene Clone |
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