Characterization of a partial amphiploid between Triticum aestivum cv. Chinese Spring and Thinopyrum intermedium ssp. trichophorum |
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| Authors: | Yang Zu-Jun Li Guang-Rong Chang Zhi-Jian Zhou Jian-Ping Ren Zheng-Long |
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| Institution: | (1) School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan, China;(2) Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan City, 611830, Sichuan, China;(3) Crop Genetic Institute of Shanxi Academy of Agricultural Sciences, Taiyuan, 030031, Shanxi, China |
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| Abstract: | A partial amphiploid, TE-3, between Triticum aestivum cv. Chinese Spring (CS) and Thinopyrum intermedium ssp. trichophorum was characterized by cytological observation, genomic in situ hybridization (GISH), seed storage protein electrophoresis and disease resistance screening. The TE-3 plants were deeply covered with pubescence, which is characteristic of the Th. intermedium ssp. trichophorum parent. Feulgen staining of the somatic metaphases revealed that the chromosome number varied from 52 to 56. TE-3 pollen mother cells (PMCs) regularly showed two to four univalents and 25 to 27 bivalents, indicating a degree of cytological instability. Giemsa-C banding showed that the Thinopyrum chromosomes in TE-3 produced strong heterochromatin bands. GISH analysis suggested that the alien chromosomes in TE-3 consisted of eight St chromosomes, four Js chromosomes, and two J genome chromosomes, as well as two St-J translocation chromosomes. Seeds storage proteins separated by acid polyacrylamide gel electrophoresis (APAGE) and sodium dodecyl sulphate – polyacrylamide gel electrophoresis (SDS-PAGE) showed that TE-3 expressed some of Th. intermedium ssp. trichophorum specific gliadin and glutenin bands. When inoculated with stripe rust and powdery mildew isolates, TE-3 expressed resistance derived from its Thinopyrum parent. It appears that TE-3 can be used as a donor source in wheat breeding programs to introduce novel variation for quality and disease resistance. |
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| Keywords: | C-banding genomic in situ hybridization partial amphiploid Thinopyrum intermedium ssp trichophorum wheat |
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