| 石斛的分子生物学鉴定--基于RAPD分析 |
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| 引用本文： | 彭锐,李泉森,李隆云.石斛的分子生物学鉴定--基于RAPD分析[J].西南农业大学学报,2004,26(4):437-440. |
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| 作者姓名： | 彭锐 李泉森 李隆云 |
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| 作者单位： | 重庆市中药研究院，重庆市中药研究院，重庆市中药研究院 重庆 400065，重庆 400065，重庆 400065 |
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| 基金项目： | 重庆市科委应用基础课题资助项目(2000-6271) |
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| 摘 要： | 利用随机扩增多态性DNA(RAPD)技术分析了15种石斛属药用植物，选用10个有效引物(10bp)扩增出99条DNA片段，用于遗传多样性分析、构建UPGMA聚类图。分析结果表明：RAPD方法能有效鉴别所试石斛属植物，其多态位点百分率为84．85％，表现出丰富的遗传多样性。
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| 关 键 词： | 石斛属 RAPD 遗传多样性 |
| 文章编号： | 1000-2642(2004)04-0437-04 |
RAPD - BASED MOLECULAR IDENTIFICATION OF DENDROBIUM SPECIES |
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| PENG Rui,LI Quan-sen,LI Long-yun.RAPD - BASED MOLECULAR IDENTIFICATION OF DENDROBIUM SPECIES[J].Journal of Southwest Agricultural University,2004,26(4):437-440. |
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| Authors: | PENG Rui LI Quan-sen LI Long-yun |
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| Abstract: | Fifteen species of the genus Dendrobium were analyzed, using RAPD (random amplified polymorphic DNA) technique. Ten decanucleotide primers (10 bp) were selected from 70 primers, and 99 bands of amplification were obtained, which were used for RAPD analysis. Based on the RAPD profile, a dendrogram of cluster analysis of the species studied was constructed according to their similarity coefficients. It is concluded that RAPD is effective in characterizing Dendrobium species. The high percentage of polymorphic loci ((84.85%)) in the species indicates that this genus is highly diverse genetically. |
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| Keywords: | Dendrobium RAPD genetic diversity |
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