| 基于高通量测序的土壤微生物群落结构对土地利用方式的响应 |
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| 引用本文： | 邓楚璇,周英,李上官,刘星科,王文娟,李西,孙凌霞,罗春兰,李向,黄玉梅.基于高通量测序的土壤微生物群落结构对土地利用方式的响应[J].四川林业科技,2021(1):16-24. |
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| 作者姓名： | 邓楚璇 周英 李上官 刘星科 王文娟 李西 孙凌霞 罗春兰 李向 黄玉梅 |
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| 作者单位： | 四川农业大学风景园林学院;彭州市规划和自然资源局 |
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| 基金项目： | 四川省教育厅重点项目(16ZA0031)。 |
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| 摘 要： | 为探究土地利用方式对土壤微生物群落的影响,对彭州市境内5种常见土地利用方式(城市绿地、农业用地、人工林地、滩涂地、撂荒地)下的土壤细菌和真菌进行了高通量测序。共检测到细菌37门106纲278目491科959属2137种,主要优势菌门为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)、绿弯菌门(Chloroflexi);真菌17门46纲118目295科627属1033种,主要优势菌门为子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、被孢霉门(Mortierellomycota)。5种土地利用模式下的土壤理化性质和土壤微生物多样性均有显著差异。农业用地的土壤微生物多样性较低,城市绿地和滩涂地具有较高的微生物多样性,而人工林地具有较低的细菌多样性和较高的真菌多样性。土壤理化性质对细菌多样性有重要影响,但对真菌影响较小。同时,土壤pH值是影响本研究微生物多样性及群落结构的主要环境因子。研究表明,杉木人工林及农作物连作模式降低了微生物多样性,而城市绿地和滩涂地为微生物提供了较稳定的生存环境,可见不同土地利用方式对土壤微生物群落结构及多样性产生了显著影响。
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| 关 键 词： | 高通量测序 土地利用方式 微生物多样性 群落结构 |
Response of Soil Microbial Community Structure to Land Use Types Based on High-throughput Sequencing Analysis |
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| DENG Chuxuan,ZHOU Ying,LI Shangguan,LIU Xingke,WANG Wenjuan,LI Xi,SUN Lingxia,LUO Chunlan,LI Xiang,HUANG Yumei.Response of Soil Microbial Community Structure to Land Use Types Based on High-throughput Sequencing Analysis[J].Journal of Sichuan Forestry Science and Technology,2021(1):16-24. |
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| Authors: | DENG Chuxuan ZHOU Ying LI Shangguan LIU Xingke WANG Wenjuan LI Xi SUN Lingxia LUO Chunlan LI Xiang HUANG Yumei |
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| Institution: | (Department of Landscape Architecture,Sichuan Agricultural University,Chengdu 611130,China;Pengzhou Planning and Natural Resources Bureau,Pengzhou 611900,China) |
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| Abstract: | In order to investigate the effects of different land use types on soil microbial community,soil bacteria and fungi under five common land use types(urban greenland,farmland,plantation woodland,tidal wetland,abandoned farmland)in Pengzhou were investigated by using high-throughput sequencing technique(MiSeq).The results showed that:(1)A total 37 phyla,106 classes,278 orders,491 families,959 genera,2137 species of soil bacteria were dectected.The main dominant phyla were Proteobacteria,Actinobacteria,Acidobacteria and Chloroflexi.There were 1033 species of soil fungi belonging to 627 genera,295 families,118 orders,46 classes,17 phyla,and the dominant phyla were Ascomycota,Basidiomycota and Mortierellomycota.(2)There were significant differences in soil physical and chemical properties and the soil microbial diversity under five land use types.Soil microbial diversity was low in farmland but higher in urban greenland and tidal wetland.While in plantation woodland,there was less bacterial diversity and more fungal diversity.Pearson correlation analysis and RDA analysis revealed that soil physical and chemical properties had strong impacts on soil bacterial diversity,but had little influence on fungi.At the same time,soil pH was the main environmental factor affecting microbial diversity and community structure in this study.The results showed that continuous Chinese fir plantation and crop cultivation reduced the microbial diversity,while urban green land and tidal wetland provided a stable living environment for soil microorganisms,which indicated that different land use types had significant impacts on soil microbial community structure and diversity. |
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| Keywords: | High-throughput sequencing Land use type Microbial diversity Community structure |
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