| 外源一氧化氮对干旱胁迫下烤烟脂膜过氧化的抑制效应 |
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| 引用本文： | 林二阁,李春光,陈孟起,李耀光,王省伟,张晓娟,肖先仪,刘英杰,喻保华,景延秋.外源一氧化氮对干旱胁迫下烤烟脂膜过氧化的抑制效应[J].中国农业科技导报,2018,20(1):55-62. |
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| 作者姓名： | 林二阁 李春光 陈孟起 李耀光 王省伟 张晓娟 肖先仪 刘英杰 喻保华 景延秋 |
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| 作者单位： | 1.河南农业大学烟草学院, 郑州450002; 2.河南中烟工业有限责任公司技术中心, 郑州 450002;3.河南省烟草公司登封市公司, 河南 登封 452470; 4.河南省烟草公司平顶山市公司, 河南 平顶山 467002; 5.江西省烟草公司赣州市公司, 江西 赣州 341000; 6.河南省烟草公司郑州市公司, 郑州 450001; 7.河南农业大学经济与管理学院, 郑州 450002 |
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| 基金项目： | 河南省烟草公司郑州市公司应用基础研究项目(ZYKJ201502);江西烟草专卖局科技项目\[赣烟科(2016)81号\]资助。 |
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| 摘 要： | 为探究一氧化氮(NO)在干旱胁迫下对烤烟脂膜过氧化的抑制效应,采用盆栽试验研究了不同浓度NO供体硝普钠(sodium nitroprusside,SNP)对干旱胁迫下烤烟叶片和根系膜透性、MDA含量、抗氧化酶活性及渗透调节物质含量的影响。结果表明:干旱胁迫下,烤烟叶片和根系膜透性,丙二醛(malondialdehyde,MDA)、可溶性蛋白、可溶性糖、脯氨酸(proline,Pro)、K+含量以及超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)活性明显提升,过氧化氢酶(catalase,CAT)显著降低;喷施0.25 mmol/L和0.50 mmol/L SNP分别使烤烟叶片、根系膜透性和MDA含量显著降低,抗氧化酶活性和渗透调节物质含量显著增加。说明外源SNP能有效提高烤烟抗氧化酶活性和渗透调节物质含量,维持细胞膜系统稳定性,缓解干旱胁迫对烤烟细胞脂膜的氧化损伤,同时SNP在缓解干旱损伤时具有浓度效应,且烤烟不同器官对SNP浓度响应不同。
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| 关 键 词： | 烤烟 一氧化氮 干旱胁迫 脂膜 抗氧化酶 |
Inhibitory Effects of Exogenous Nitric Oxide on Lipid Peroxidation of Flue-Cured Tobacco Under Drought Stress |
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| LIN Erge,LI Chunguang,CHEN Mengqi,LI Yaoguang,WANG Xingwei,ZHANG Xiaojuan,XIAO Xianyi,LIU Yingjie,YU Baohua,JING Yanqiu.Inhibitory Effects of Exogenous Nitric Oxide on Lipid Peroxidation of Flue-Cured Tobacco Under Drought Stress[J].Journal of Agricultural Science and Technology,2018,20(1):55-62. |
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| Authors: | LIN Erge LI Chunguang CHEN Mengqi LI Yaoguang WANG Xingwei ZHANG Xiaojuan XIAO Xianyi LIU Yingjie YU Baohua JING Yanqiu |
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| Abstract: | In order to explore the inhibitory effects of nitric oxide on lipid peroxidation in flue-cured tobacco under drought stress, the pot experiment was carried out to investigate the effects of different concentrations of exogenous NO donor (sodium nitroprusside, SNP) on the membrane permeability of leaf and root system of flue-cured tobacco, MDA content, antioxidant enzyme activity and content of osmotic adjustment substances under drought stress. The results showed that membrane permeability of leaf and root system, the contents of MDA, soluble protein, soluble sugar, proline (Pro) and K+, and the activities of superoxide dismutase (SOD) and catalase (POD) of the leaves and roots of tobacco increased significantly under drought stress, while the content of catalase (CAT) decreased significantly. The membrane permeability of leaf and root system and MDA content decreased significantly after spraying 0.25 mmol/L and 0.5 mmol/L, while the activity of antioxidant enzymes and content of osmotic adjustment substances increased significantly. This indicated that exogenous SNP could effectively improve the activity of antioxidant enzymes and content of osmotic adjustment substances, maintain the stability of cell membrane, and alleviate the oxidative damage of lipid membranes of flue cured tobacco under drought stress. It also showed that the concentration effect of SNP on alleviating drought damage, and different organs of flue-cured tobacco had different response to SNP concentration. |
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| Keywords: | flue-cured tobacco nitric oxide drought stress lipid antioxidant enzyme |
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