| 牛ACTA1基因的生物信息学分析 |
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| 引用本文： | 张馨月,田万年.牛ACTA1基因的生物信息学分析[J].畜牧与饲料科学,2018,39(3):17-17. |
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| 作者姓名： | 张馨月 田万年 |
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| 作者单位： | [1]吉林农业科技学院动物科技学院,吉林吉林132101 |
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| 基金项目： | 吉林农业科技学院大学生科技创新项目(吉农院合字[2016]第066号)。 |
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| 摘 要： | 应用RT-PCR方法克隆牛ACTA1基因,利用生物信息软件对该基因进行生物信息学分析。结果表明:牛ACTA1基因CDS为1134bp,编码377个氨基酸;拓扑预测表明,ACTA1编码的蛋白质可能存在4个酪蛋白激酶Ⅱ磷酸化位点,8个豆蔻酰化位点,含有1个ACTIN保守的结构域。研究结果为进一步了解牛ACTA1基因调控肉质性状的分子机理提供了有益参考。
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Bioinformatical Analysis of ACTA1 Gene in Cattle |
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| Abstract: | In the present study,the ACTA1 gene of Yanbian Yellow cattle was cloned by RT-PCR assay and subsequently bioinformatically analyzed with bioinformatics analysis software.The results showed that the CDS of ACTA1 gene was composed of 1 134 nucleotides which encoded 377 amino acid residues;topological prediction revealed that the protein encoded by ACTA1 gene had four casein kinaseⅡphosphorylation sites,eight N-myristoylation sites,and one ACTIN conserved domain.Results of this study provide references for understanding the mechanism underling the regulative effect of ACTA1 gene on meat quality in bovine. |
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