| 植物单宁对烟草花叶病毒的抑制活性 |
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| 引用本文： | 刘国坤,吴祖建,谢联辉,林奇英,陈启建.植物单宁对烟草花叶病毒的抑制活性[J].福建农林大学学报(自然科学版),2003,32(3):292-295. |
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| 作者姓名： | 刘国坤 吴祖建 谢联辉 林奇英 陈启建 |
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| 作者单位： | 福建农林大学植物病毒研究所,福建,福州,350002 |
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| 基金项目： | 福建省科技厅重点资助项目(99-Z-193). |
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| 摘 要： | 测试了从11种植物提取的单宁对烟草花叶病毒(TMV)的抑制活性.结果表明:(1)任一植物的单宁质量浓度为5.000或2.500mg·mL-1时,与TMV混合10min后接种,其抑制率均达80%以上.(2)单宁质量浓度为5.000mg·mL-1时,与TMV混合后立即接种,其抑制率也均达80%以上,表明单宁与病毒粒体作用时间很短.(3)TMV与单宁混合,透析3d后,可恢复大部分侵染力;混合3d但未作透析处理,与混合10min的处理相比,抑制率下降,表明部分植物的单宁体外稳定性可能较差,与TMV粒体作用能力下降.(4)这11种植物的单宁对TMV体内抑制复制效果不明显.(5)大飞扬、杠板归、虎杖3种植物的单宁,在接种前先喷施心叶烟再接种TMV,可抑制病毒的初侵染;在接种前先喷施普通烟草K326再接种TMV,烟株发病期推迟3-8d.
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| 关 键 词： | 植物提取物 单宁 烟草花叶病毒 抑制 |
| 文章编号： | 1006-7817(2003)03-0292-04 |
| 修稿时间： | 2003年2月25日 |
The inhibition of infectivity of Tobacco mosaic virus by tannins extracted from plants |
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| Abstract: | The anti TMV activities of tannins extracted from 11 kinds of plants were determined. Infectivity of Tobacco mosaic virus (TMV) on Nicotiana glutinosa was strongly inhibited (more than 80%) by tannins (5.000 or 2.500 mg·mL-1) extracted from each kind of plants incubated with TMV for 10 mins. Strong inhibition (more 80%) was also showed when leaves were inoculated with TMV after incubated immediately with tannins at 5.000 mg·mL-1. The infectivity of TMV was recovered by dialysis for 3 days from incubations in vitro with tannins, but inhibition was reduced for tannins extracted from some kinds of plant after incubated with TMV for 3 days. Tannins of all plants did not affect multiplication of TMV in infected leaf discs. Tannins extracted form Euphorbia hirta, Polygonum perfoliatum, P.cuspidatum reduced infectivity when applied immediately on N.glutinosa before TMV inoculation. It also retarded symptom expression for 3-8 days in first leaves of N.tobacum cv.K326 when applied immediately before TMV inoculation. |
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| Keywords: | plant extracts tannins Tobacco mosaic virus inhibition |
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