| 产肠毒素大肠杆菌F5菌毛基因的克隆与表达 |
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| 引用本文： | 于迪,付海兵,丁琳,师东方,单安山.产肠毒素大肠杆菌F5菌毛基因的克隆与表达[J].东北农业大学学报,2008,39(6). |
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| 作者姓名： | 于迪 付海兵 丁琳 师东方 单安山 |
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| 作者单位： | 1. 东北农业大学动物医学学院,哈尔滨,150030
2. 东北农业大学动物科学技术学院,哈尔滨,150030 |
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| 基金项目： | 东北农业大学校长基金，黑龙江省科技攻关项目，黑龙江省博士后资助项目 |
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| 摘 要： | 应用PCR方法从产肠毒素性大肠杆菌(ETEC)中扩增出不含信号肽序列的F5菌毛抗原基因片段,克隆测序后将其连接到大肠杆菌表达载体pET-30a(+)中,转化工程菌BL21,经IPTG诱导表达。经SDS-PAGE和Western Blot分析,重组蛋白在BL21中得到表达并与F5菌毛单因子血清发生明显反应。
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| 关 键 词： | 产肠毒素性大肠杆菌 F5菌毛 基因克隆 蛋白表达 |
Clone and expression of pili subunit gene from adhesion F5 of the enterotoxigenic Escherichia coli(ETEC) |
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| YU Di,FU Haibing,DING Lin,SHI Dongfang,SHAN Anshan.Clone and expression of pili subunit gene from adhesion F5 of the enterotoxigenic Escherichia coli(ETEC)[J].Journal of Northeast Agricultural University,2008,39(6). |
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| Authors: | YU Di FU Haibing DING Lin SHI Dongfang SHAN Anshan |
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| Abstract: | A F5 fimbrial subunit gene without signal peptide sequence was amplified by PCR from enterotoxigenic E.coli.After cloning and sequencing,the fragment was inserted into an E.coli expression vector pET-30a( ),then the recombinant plasmid was transferred into E.coli BL21,and E.coli BL21( F5) obtained.Cultured and induced with IPTG,the expression of target protein was determined by SDS-PAGE and Western Blot.It was found that the expressed proteins could obviously react with antiserum against the single factor of F5 pili subunit proteins. |
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| Keywords: | ETEC F5 fimbrial gene cloning protein expression |
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