In vitro induction of tetraploid ginger (Zingiber officinale Roscoe) and its pollen fertility and germinability |
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| Institution: | 1. Institute of Tropical Agriculture and Forestry, Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Hainan University, Haikou, Hainan, 570228, China;2. Tropical Crops Genetic Resources Institute,CATAS, Danzhou, Hainan, 571737, China;1. College of Agriculture and Food Science, Zhejiang A&F University, Zhejiang Provincial Agricultural Product Quality Improvement Technology Research Key Laboratory, Biology Center of Zhejiang A& F University, Wushu Street 666, Lin’an, Hangzhou, Zhejiang, 311300, PR China;2. Department of Health and Agriculture, Hangzhou Wanxiang Polytechnic, Huajiang Road 3, Hangzhou, Zhejiang, 310023, PR China;1. The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China;2. College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;3. Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, China;4. College of Horticulture, South China Agricultural University, Guangzhou 510642, China;1. School of BioSciences, The University of Melbourne, Parkville, Victoria, 3010, Australia;2. School of Biological Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia;3. School of Ecosystem and Forest Sciences, The University of Melbourne, Parkville, Victoria, 3010, Australia;1. Instituto de Genética “Ewald A. Favret”, Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina;2. Instituto de Recursos Biológicos, Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina;3. Cátedra de Genética, Facultad de Agronomía, Universidad de Buenos Aires (FAUBA), Ciudad Autónoma de Buenos Aires, Argentina;4. Cátedra de Farmacognosia, IQUIMEFA—CONICET, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires (FFyB-UBA), Ciudad Autónoma de Buenos Aires, Argentina |
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| Abstract: | In vitro induction of tetraploid ginger (Zingiber officinale Roscoe) and its pollen fertility and germinability were investigated. The growth of shoot tip cultures on agar MS medium containing 2.0 mg l?1 BA was greater than that of similar cultures in liquid MS medium with the same BA concentration. In liquid medium, the combinations of 0.5, 1.0, or 2.0 mg l?1 BA with 0.05 mg l?1 NAA tended to enhance the growth of the cultures. The efficiency of tetraploid induction was assessed by treating shoot tip explants on agar or in liquid MS medium containing 2.0 mg l?1 BA, 0.05 mg l?1 NAA, and 0.2% (w/v) colchicine for 4, 8, 12, and 14 days. The total number of tetraploids induced on solid medium was 18, but only five in liquid medium. On both media, the colchicine treatment for 8 days gave the maximum level of tetraploid induction. Therefore, it was found that the treatment of shoot tip explants on agar medium containing 2.0 mg l?1 BA, 0.05 mg l?1 NAA, and 0.2% (w/v) colchicine for 8 days was the most efficient way of inducing tetraploid ginger. Induced tetraploid strains, ‘4× Kintoki’, ‘4× Sanshu’, and ‘4× Philippine cebu 1’, had higher pollen fertility and germinability than the diploid counterparts, i.e., in the diploid strains, pollen fertility ranged from 0.3 to 6.2% and the germination rate from 0.0 to 0.1%, while in the tetraploid strains, pollen fertility ranged from 27.4 to 74.2% and the germination rate from 4.8 to 12.9%. |
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