Shoot induction and plant regeneration from receptacle tissues of Lilium longiflorum |
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| Institution: | 1. Departamento de Química Inorgánica, Instituto de Química, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, CDMX 04510, Mexico;2. Departamento de Química de Radiaciones y Radioquímica, Instituto de Ciencias Nucleares, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, CDMX 04510, Mexico;3. Centro Conjunto de Investigación en Química Sustentable UAEMex-UNAM, Instituto de Química, Universidad Nacional Autónoma de México, Carretera Toluca-Atlacomulco Km 14.5, Toluca, Estado de México 50200, Mexico;4. Departamento de Microbiología y Parasitología, Facultad de Biología CIBUS, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain |
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| Abstract: | An efficient system has been developed for the in vitro plant regeneration of Lilium longiflorum Thunb. by culturing receptacle sections from flower buds. The sections were cultured on one-half MS medium plus 30 g l?1 sucrose, 8 g l?1 agar, 5.4 μM NAA or 4.9 μM IBA plus 2.2 μM BAP. A section size of 3–4 mm was found to be optimal. After 60 days an average of 41 shoots were formed per explant. More vigorous shoots were obtained by subculturing on hormone-free medium with 20 g l?1 sucrose. Rooting occurred on one-half MS medium with 1.1 μM NAA. Rooted plants were hardened-off in a greenhouse for two months, and normal flowering plants were produced. |
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