| 基于18S rRNA和HSP70基因序列的隐孢子虫种系发育分析 |
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| 引用本文： | 王进产,菅复春,张龙现,宁长申,闫文朝,孙铭飞,仇书兴,卢庆斌.基于18S rRNA和HSP70基因序列的隐孢子虫种系发育分析[J].畜牧兽医学报,2007,38(9):947-953. |
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| 作者姓名： | 王进产 菅复春 张龙现 宁长申 闫文朝 孙铭飞 仇书兴 卢庆斌 |
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| 作者单位： | 河南农业大学牧医工程学院,郑州,450002 |
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| 基金项目： | 国家自然科学基金资助项目（30371079）;河南省高校创新人才培养工程项目（豫教高[2004]294） |
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| 摘 要： | 为阐明河南区域隐孢子虫分子流行病学特点,用PCR技术扩增分离虫株的18S rRNA基因全序列和HSP70基因序列,并对扩增片段进行测序。用PAUP 4.0和TREEPUZZLE 4.1构建进化树,试图从分子水平证明河南省不同地区不同宿主来源隐孢子虫的遗传特征,以阐明隐孢子虫病的分子流行病学特点。通过18S rRNA基因全序列和HSP70基因序列分析,其结果：河南人源隐孢子虫分离株为Cryptosporidium parvum鼠基因型;河南鹿源隐孢子虫分离株为C. parvum鹿基因型;河南猪源隐孢子虫的2个分离株均为C. parvum猪基因I型,即C. suis;河南鹌鹑源的隐孢子虫2个分离株分别为C. baileyi和C. meleagridis;河南乌鸡源隐孢子虫和鸵鸟源隐孢子虫分离株均为C. baileyi;河南牛源隐孢子虫分离株为C.andersoni。
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| 关 键 词： | 隐孢子虫 HSP70 18S rRNA 种系发育 |
| 文章编号： | 0366-6964（2007）09-0947-07 |
| 修稿时间： | 2006-10-24 |
Phylogentic Analysis of the Cryptosporidium Based on 18S rRNA Gene and HSP70 Gene Sequences |
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| WANG Jin-chan,JIAN Fu-chun,ZHANG Long-xian,NING Chang-shen,YAN Wen-chao,SUN Ming-fei,QIU Shu-xing,LU Qing-bin.Phylogentic Analysis of the Cryptosporidium Based on 18S rRNA Gene and HSP70 Gene Sequences[J].Acta Veterinaria et Zootechnica Sinica,2007,38(9):947-953. |
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| Authors: | WANG Jin-chan JIAN Fu-chun ZHANG Long-xian NING Chang-shen YAN Wen-chao SUN Ming-fei QIU Shu-xing LU Qing-bin |
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| Institution: | College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China |
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| Abstract: | In order to investigate species or genotype of the Cryptosoridia isolates that derived from different hosts in Henan province,the author amplified the complete sequences of the small-subunit rRNA(18S rRNA) gene and the 70 ku heat shock protein(HSP70) gene.Phylogentic analyses were performed using PAUP 4.0 and TREEPUZZLE 4.1.Species and genotypes of all Cryptosporidium isolates that derived from livestock,poultry and human in Henan were determined for understanding the mechanism of Cryptosporidium molecular epidemiology.We characterized the 18S rRNA and HSP70 genes of the isolates.Results of the phylogenetic analysis revealed that the Cryptosporidia human strain is Cryptosporidium parvum mouse genotype;the Cryptosporidia deer(wapiti) strain is C.parvum deer genotype,the two Cryptosporidia swine strains both are C.suis;the two Cryptosoridia quail strains belong to different species,one is C.bailey,the other is C.meleagridis;both the other Cryptosporidia birds strains(isolated from chicken and ostrich) are C.baileyi;the Cryptospridia isolates from cattle is C.andersoni. |
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| Keywords: | Cryptosporidium HSP70 18S rRNA phylogentic development |
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