| 含ACC脱氨酶活性的复合菌株提高苜蓿抗盐碱能力研究 |
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| 引用本文： | 刘冠一,刘艳玲,刘博文,刘莎莎,郭长虹.含ACC脱氨酶活性的复合菌株提高苜蓿抗盐碱能力研究[J].核农学报,2017,31(5). |
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| 作者姓名： | 刘冠一 刘艳玲 刘博文 刘莎莎 郭长虹 |
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| 作者单位： | 哈尔滨师范大学生命科学与技术学院/分子细胞遗传与遗传育种黑龙江省重点实验室,黑龙江哈尔滨,150025 |
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| 基金项目： | 国家自然科学基金，黑龙江省科技攻关项目，哈尔滨师范大学研究生创新基金 |
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| 摘 要： | 为探究含ACC脱氨酶活性的复合菌株对盐碱胁迫下紫花苜蓿的促生效果,以3株含ACC脱氨酶活性的植物根际促生菌拜式不动杆菌Y-2(Acinetobacter beijerinckii)、粘质沙雷氏菌BC-3(Serratia marcescens)和产气肠杆菌CE-1(Enterobacter aerogenes)为供试菌株,通过菌株拮抗反应测试,筛选可以混合使用的菌株,测定单菌株和复合菌株的耐盐碱特性和促生特性,并进行盐碱土盆栽试验,评价其对紫花苜蓿的促生效果。结果表明,复合菌株Y-2+CE-1和BC-3+CE-1间无拮抗反应,可以混合使用,在p H值为10和盐浓度为7%的条件下生长良好。单菌株和复合菌株均具有ACC脱氨酶活性、分泌植物生长素(IAA)能力和溶磷能力,且复合菌株的促生特性优于单菌株。此外,接种单菌株和复合菌株均能不同程度地促进紫花苜蓿的生长,其中复合菌株Y-2+CE-1和BC-3+CE-1促生效果显著,与对照相比,株高、根长、地上干重、根干重分别提高20.3%和20%、51.4%和45.9%、47%和36.4%、68.8%和52%,且促生效果优于单菌株。可见,含ACC脱氨酶活性的复合菌株能缓解盐碱胁迫,对紫花苜蓿具有明显的促生效果。本研究结果为研制复合菌肥,治理和利用盐碱地奠定了理论基础。
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| 关 键 词： | 盐碱胁迫 植物根际促生菌 ACC脱氨酶 互作 紫花苜蓿 |
Research on Enhanced Alfalfa Tolerance to Saline-alkali Stress by Strain Combinations Containing ACC Deaminase |
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| LIU Guanyi,LIU Yanling,LIU Bowen,LIU Shasha,GUO Changhong.Research on Enhanced Alfalfa Tolerance to Saline-alkali Stress by Strain Combinations Containing ACC Deaminase[J].Acta Agriculturae Nucleatae Sinica,2017,31(5). |
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| Authors: | LIU Guanyi LIU Yanling LIU Bowen LIU Shasha GUO Changhong |
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| Abstract: | In order to investigate the promoting effect of alfalfa using strain combinations containing ACC deaminase activity under saline-alkali stress,co-inoculated plant growth promoting rhizobacteria Y-2 (Acinetobacter beijeritckii),BC-3 (Serratia marcescens) and CE-1 (Enterobacter aerogenes) combinations containing ACC deaminase were selected by antagonistic reaction.The characteristics of salt-alkali tolerance and growth-promoting of single and combinations strains were evaluated,as well as evaluating the effect of promoting on the growth of alfalfa by pot experiment.Results showed that there were no antagonistic reactions between Y-2 + CE-1 and BC-3 + CE-1 strain combinations,and the two strain combinations could be mixed and grew well under the condition of pH10 and 7％ NaCl.Both single stain and combinations could synthesize ACC deaminase,secreat IAA (Indole-3-acetic acid) and solubilize phosphate.The growth-promoting of strain combinations were better than single strain.In addition,inoculation of single and combination strains could promote alfalfa growth in different extent,compared with control,the remarkable effects of plant growth promotion were found in co-inoculated strain combinations Y-2 + CE-1 and BC-3 + CE-1,the plant height were increased by 20.3％ and 20％,respectively,the root length were increased by 51.4％ and 45.9％ respectively,dry weight of shoot were increased by 47％ and 36.4％ respectively,the dry weight of root were increased by 68.8％ and 52％ respectively,and better than alfalfa inoculated with single strain.The data obtained in our study suggest that strain combinations containing ACC deaminase activity can alleviate saline-alkaline stress,and promote the growth of alfalfa significantly.This paper established the theoretical foundation for the development of compound fertilizer,governance and utilization of saline-alkalive soil. |
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| Keywords: | saline-alkaline stress plant growth promoting rhizobacteria ACC deaminase interaction alfalfa |
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