| γ射线辐照降解木聚糖的机理研究 |
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| 引用本文： | 武小芬,陈亮,陈静萍,苏小军,齐慧,王克勤,邓明.γ射线辐照降解木聚糖的机理研究[J].核农学报,2017,31(5). |
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| 作者姓名： | 武小芬 陈亮 陈静萍 苏小军 齐慧 王克勤 邓明 |
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| 作者单位： | 1. 湖南省农业科学院湖南省核农学与航天育种研究所/湖南省农业生物辐照工程技术研究中心,湖南长沙,410125;2. 湖南省农业科学院湖南省农业生物技术研究中心,湖南长沙,410125;3. 湖南农业大学湖南省种质创新与资源利用重点实验室,湖南长沙,410128 |
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| 基金项目： | 公益性行业(农业)科研专项 |
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| 摘 要： | 为了探讨γ射线辐照降解半纤维素的机理,以木聚糖为模型化合物,经0、200、400、600、800、1 000、1 200 k Gy剂量的辐照后,采用扫描电镜(SEM)、傅里叶拉曼光谱(FT-Raman)、气相色谱质谱联用仪(GC-MS)研究其表观形貌结构、分子结构及降解产物的变化特征。结果表明,γ射线辐照处理后木聚糖表观形貌结构发生破坏,多孔性增加;随着辐照剂量升高,木聚糖3 354 cm~(-1)处拉曼光谱峰强度降低且峰位发生红移,1 122 cm~(-1)和1 093 cm~(-1)处拉曼光谱峰强度逐渐减弱,表明木聚糖分子中的氢键、糖苷键发生破坏;木聚糖降解产物的GC-MS分析表明,辐照处理后木聚糖乙酸乙酯提取液中共检测到18种脂肪酸类化合物、5种糖类化合物和2种呋喃类化合物,其相对含量分别在1 200、800和1 200 k Gy达到最大值(16.59%、1.46%和1.03%)。本研究结果为木聚糖γ射线辐照降解产物全利用提供了支持。
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| 关 键 词： | γ射线辐照 木聚糖 结构 降解产物 降解机理 |
Study on the Degradation Mechanism of Xylan by Gamma Rays Irradiation |
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| WU Xiaofen,CHEN Liang,CHEN Jingping,SU Xiaojun,QI Hui,WANG Keqin,DENG Ming.Study on the Degradation Mechanism of Xylan by Gamma Rays Irradiation[J].Acta Agriculturae Nucleatae Sinica,2017,31(5). |
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| Authors: | WU Xiaofen CHEN Liang CHEN Jingping SU Xiaojun QI Hui WANG Keqin DENG Ming |
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| Abstract: | To understand the mechanism of hemicellulose degradation by irradiation,xylan was used as a model compound in this study.The characteristic difference of apparent topography,molecular structure and degradation products of xylan after γ-ray irradiation with different doses of 0,200,400,600,800,1 000,1 200 kGy were analyzed by Scanning Electron Microscopy (SEM),Fourier Transform Raman Spectroscopy (FT-Raman),and Gas Chromatography Mass Spectrometry(GC-MS).The results showed that the apparent topography in xylan was damaged and also increase in porosity of xylan.The intensity of Raman Spectra of 3 354 cm-1 decreased with the location of peak showing a red shift,and the intensity of Raman Spectra of 1 122 cm-1 and 1 093 cm-1 decreased with the increasing dose of γ irradiation,which illuminated the hydrogen bonds and glycosidic bonds of xylan being fractured,respectively.Analyzed degradation products from ethyl acetate extract of xylan after irradiation by GC-MS showed 18 kinds of fatty acid compounds,5 kinds of saccharides and 2 kinds of furan compounds,and the relative contents of these three compounds group reached the maximum level of 16.59％,1.46％ and 1.03％,at the irradiation dose of 1 200 kGy,800 kGy and 1 200 kGy,respectively.This study provides support on degradation mechanism of lignocellulose and comprehensive utilization of xylan after γ-irradiation pretreatment. |
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| Keywords: | gamma rays irradiation xylan structure degradation products degradation mechanism |
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