| 花叶性状在油菜杂交种纯度控制中的应用 |
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| 引用本文： | 倪西源,黄吉祥,柳寒,潘兵,赵坚义.花叶性状在油菜杂交种纯度控制中的应用[J].核农学报,2017,31(6). |
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| 作者姓名： | 倪西源 黄吉祥 柳寒 潘兵 赵坚义 |
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| 作者单位： | 1. 浙江省农业科学院作物与核技术利用研究所/植物有害生物防控国家重点实验室省部共建培育基地,浙江杭州,310021;2. 浙江大学农业与生物技术学院作物科学研究所,浙江杭州,310029 |
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| 基金项目： | 国家重点研发计划项目，浙江省农业新品种选育重大科技专项，浙江省植物有害生物防控重点实验室—省部共建国家重点实验室培育基地基金 |
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| 摘 要： | 为了将花叶性状转入到甘蓝型油菜隐性上位互作核不育系统中用于控制杂交种的纯度,在油菜隐性上位互作核不育纯合不育株与花叶型油菜品系羽叶87的杂交后代中,利用2个与育性基因连锁的分子标记辅助筛选两型系和临保系基因型单株,获得具有花叶性状的两型系和临保系。结果表明,可通过F_2世代选育法、系谱法和回交法3种途径选育花叶两型系和花叶临保系;在花叶两型系和花叶临保系的繁殖以及花叶全不育系的配制过程中,可通过去除非花叶杂株来确保纯度。因此,花叶性状作为一个理想的形态标记,可以应用于油菜的杂种生产来控制种子的纯度。本研究结果为杂交种的纯度控制提供了一条简捷且有效的途径。
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| 关 键 词： | 甘蓝型油菜 形态标记 花叶 隐性上位互作核不育 杂种纯度 |
Application of Lobed-leaf Trait in Controlling Hybrid Purity of Rapeseed |
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| NI Xiyuan,HUANG Jixiang,LIU Han,PAN Bing,ZHAO Jianyi.Application of Lobed-leaf Trait in Controlling Hybrid Purity of Rapeseed[J].Acta Agriculturae Nucleatae Sinica,2017,31(6). |
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| Authors: | NI Xiyuan HUANG Jixiang LIU Han PAN Bing ZHAO Jianyi |
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| Abstract: | In order to introduce the lobed-leaf trait into a recessive epistatic genic male sterility system of rapeseed (Brassica napus L.) to control the purity of hybrid seeds,homozygous male sterile plant was crossed with lobed-leaf line Yuye 87,then lobed-leaf two-type lines and lobed-leaf temporary maintainer lines were selected based on marker assisted selection (MAS) in the progeny populations,and two markers linked to the sterility genes were used in MAS.The results showed that two-type lines and temporary maintainer lines with lobed leaves could be bred by three approaches including selection in F2 population,pedigree method and backcross method,i,e.lobed-leaf trait could be used as a morphological marker to ensure the purity of two-type lines,lobed-leaf temporary maintainer lines and male sterility lines by removing the plants with normal leaf shape in the process of multiplication,and the purity of hybrid seeds can be controlled.Therefore,lobed-leaf trait is an ideal morphological marker for controlling seed purity in hybrid production of rapeseed.The results provided a simple and effective way to control the hybrid purity of rapeseed. |
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| Keywords: | Brassica napus L morphological marker lobed-leaf recessive epistatic genic male sterile hybrid purity |
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