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入侵植物假臭草和胜红蓟提取物的抑菌活性研究
引用本文:李书明,陈雅婷,王奇志.入侵植物假臭草和胜红蓟提取物的抑菌活性研究[J].核农学报,2017,31(9).
作者姓名:李书明  陈雅婷  王奇志
作者单位:华侨大学化工学院生物工程与技术系,福建 厦门,361021
基金项目:福建省农业重点自然科学基金项目,泉州市校(院)地协同创新专项(重点)项目,华侨大学研究生科研创新能力培育计划资助项目
摘    要:假臭草和胜红蓟是我国南方常见的入侵植物,为了防治和利用其开发植物源抑菌剂,本试验采用牛津杯法检测了不同溶剂(70%乙醇、95%乙醇、石油醚和乙酸乙酯)提取物对28种病原微生物的抑菌活性,并评估了不同器官部位提取物的抑菌活性。结果表明,不同溶剂提取物抑菌活性有差异,假臭草70%乙醇提取物对金黄色葡萄球菌(Staphylococcus aureus)抑制活性最强,抑菌圈直径为26.28±0.75mm;胜红蓟95%乙醇提取物对腐生葡萄球菌(Staphylococcus epidermidi)抑制活性最好,抑菌圈直径为17.31±0.46 mm。此外,假臭草和胜红蓟70%乙醇不同部位提取物对金黄色葡萄球菌(Staphylococcus aureus)的抑菌作用表现为叶部提取物(22.31±0.34 mm和19.52±0.28 mm)茎部提取物(22.25±0.65 mm和12.75±0.45 mm)根部提取物(11.78±0.69 mm和9.05±0.53 mm)。假臭草提取物对12种细菌和1种真菌黑曲霉菌(Aspergillus niger)具有抑制效果;胜红蓟提取物对9种细菌具有抑制效果。本研究结果为进一步研发具有潜力的植物源抑菌剂奠定了理论基础。

关 键 词:入侵植物  假臭草  胜红蓟  抑菌活性

Antibacterial Activity of Extracts on Invasive Plant of Praxelis Clematidea (Griseb.)R.M.King & H.Rob.and Ageratum conyzoides L.
LI Shuming,CHEN Yating,WANG Qizhi.Antibacterial Activity of Extracts on Invasive Plant of Praxelis Clematidea (Griseb.)R.M.King & H.Rob.and Ageratum conyzoides L.[J].Acta Agriculturae Nucleatae Sinica,2017,31(9).
Authors:LI Shuming  CHEN Yating  WANG Qizhi
Abstract:Praxelis clematidea(Griseb.)R.M.King & H.Rob.and Ageratum conyzoides L.are invasive species widely distributed in southern China.To prevent these plants dispersal and use of them as botanical fungicides,their extracts were used against 28 species of pathogenic bacteria using the Oxford cup method.Plants were extracted with 70% ethanol,95% ethanol,petroleum ether and ethyl acetate.And the antibacterial properties of extracts from different organs were evaluated.The results showed that the antibacterial activity of these various extracts were different.The 70% ethanol extract of P.clematidea had the best antibacterial activity against Staphylococcus aureus,with an inhibition zone diameter of 26.28 ± 0.75 mm,whereas the 95% ethanol extract of A.conyzoides had the best antibacterial activity against S.aureus,with an inhibition zone diameter of 17.31 ± 0.46 mm.A comparison of 70% ethanol extracts of leaves,stems and roots from both plant species against S.aureus showed that the antibacterial activity of leaf extracts with 22.31 ± 0.34 mm and 19.52 ± 0.28 mm for P.clematidea and A.conyzoides,respectively were the best,followed by the stem extracts,with antibacterial activity of 22.25 ± 0.65 mm and 12.75 ± 0.45 mm,and root antibacterial activity of 11.78 ± 0.69 mm and 9.05 ± 0.53 mm.Furthermore,P.clematidea extracts showed antibacterial activity against 12 different bacterial strains and the fungus Aspergillus niger,while A.conyzoides extracts showed antibacterial activity against nine bacterial strains.These studies demonstrate the potential of these plants in producing botanical fungicides.
Keywords:invasive plant  Praxelis clematidea  Ageratum conyzoides L    antibacterial activity
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