Genetic Diversity in Accessions of Wild Rice Oryza granulata from South and Southeast Asia |
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| Authors: | Wei Qian Song Ge De-yuan Hong |
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| Institution: | (1) Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences>, Beijing, 100093, P. R. China;(2) National Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100080, P. R. China |
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| Abstract: | Oryza granulata, an upland wild rice species, represents an unique germplasm for possessing abilities of tolerance to shade and drought,
immune to bacterial blight and resistance to brown planthopper. Although low degree of genetic variability has been revealed
within its populations, little genetic information at the species level is available in determining rational conservation
strategies. Here we used dominant DNA marker random amplified polymorphism DNA (RAPD) to assess the genetic variability among
23 accessions of O. granulata that collected from main distribution areas worldwide. Twenty decamer primers generated a total of 243 bands, with 83.5%
of them (203 bands) being polymorphic. Calculation of Shannon index of diversity revealed an average value of 0.42 ± 0.25,
indicating that O. granulata maintains a relatively high degree of genetic diversity on the species level. Analysis of genetic dissimilarity (GD) showed
that genetic differentiation occurred among studied accessions, which supports the feasibility of current ex situ conservation strategies. We also suggested that information based on population studies, which could be achieved by international
co-operation, is needed to conserve this widespread germplasm more effectively. |
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| Keywords: | Conservation Genetic diversity Oryza granulata RAPD markers Wild rice |
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