The use of <Emphasis Type="Italic">Vp1</Emphasis> in real time RT-PCR to select for pre-harvest sprouting tolerance in triticale |
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| Authors: | Sarah De Laethauwer Dirk Reheul Jan De Riek Geert Haesaert |
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| Institution: | (1) Faculty of Biosciences and Landscape Architecture, University College Ghent and University Ghent, Voskenslaan 270, 9000 Ghent, Belgium;(2) Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium;(3) Institute for Agricultural and Fisheries Research, Plant Unit, Caritasstraat 21, 9090 Melle, Belgium |
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| Abstract: | In spite of the availability of laboratory and field tests there is still a major problem to select pre-harvest sprouting
(PHS) tolerant triticale varieties in a reliable, field-independent way. One approach to minimize the influence of environmental
conditions and physio-morphological traits on PHS detection is using molecular genetic tools. The ‘viviparous’ Vp1 gene has been repeatedly described to play an important role in dormancy in wheat. A quantitative RT-PCR assay based on the
expression of the Vp1 gene has been developed. Specific primers were designed for detecting Vp1 in both wheat and triticale. The expression levels of Vp1 were normalized using reference genes and relatively quantified with the comparative Ct-method. However, the first results indicate that the achieved Vp1 expression levels at 50 days post anthesis are not useful to select for PHS tolerance, both in wheat and triticale. This
negative outcome so far is possibly due to the existence of several splicing events or to the late assaying moment in the
kernel development, when Vp1 expression is found to be low. |
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| Keywords: | Triticale Wheat Vp1 gene Real time RT-PCR Pre-harvest sprouting |
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