| 降解茶粕高效纤维素酶的菌株的筛选 |
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| 引用本文： | 李君君,文汉.降解茶粕高效纤维素酶的菌株的筛选[J].中国农学通报,2010,26(16):31-35. |
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| 作者姓名： | 李君君 文汉 |
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| 作者单位： | 安徽农业大学生命科学学院,合肥,230001 |
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| 摘 要： | 为筛选降解茶粕高效纤维素酶的菌株提供依据。从土壤、垃圾以及腐烂的树叶中通过刚果红培养基、羧甲基纤维素培养基等培养基初筛出6株菌株L-1、L-3、L-4、L-5、L-6、L-2。然后用这6株菌种对以油茶饼粕为主要营养成分的固态培养基进行单菌发酵。先比较固态发酵时菌种的内切酶酶活得出L-1、L-3、L-4等3种菌株可做为高效降解茶粕纤维素的备选菌株,通过3种菌株的外切酶酶活、内切酶酶活、总酶活的显著性分析得知,L-3菌株的3种酶活值分别依次可达到1701.7999U、1880.0634U、1798.2334U,且其显著性高于其他的菌株,是产降解茶粕高效纤维素酶的最佳菌株,经鉴定该种菌株属于青霉属。
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| 关 键 词： | 红小豆 红小豆 品种资源 数量性状 遗传变异 |
| 收稿时间： | 2010/3/19 0:00:00 |
| 修稿时间： | 2010/4/30 0:00:00 |
Screening the strains of producing efficient cellulase which can efficiently decompose the cellulose of Camellia cake |
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| Li Junjun,Wen Han.Screening the strains of producing efficient cellulase which can efficiently decompose the cellulose of Camellia cake[J].Chinese Agricultural Science Bulletin,2010,26(16):31-35. |
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| Authors: | Li Junjun Wen Han |
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| Institution: | (Anhui Agricultural University School of Life Sciences,Hefei 230001) |
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| Abstract: | The aim of the study was to provide foundation for screening the strains of producing efficient cellulase which can efficiently decompose the cellulose of camellia cake.From the soil,garbage and rotting leaves through such as Congo red medium,carboxymethyl cellulose medium and so on mediums screened out of L-1,L-3,L-4,L-5,L-6,L-2 6 strains.Then used those six strains to individually ferment the medium which was composed of Camellia cake as the main nutritional components.By comparing the solid-state fermentation of enzyme activity,could know that L-1,L-3,L-4 three kinds of strains can be used as alternate strains which could efficiently decompose the cellulose of Camellia cake.And by comparing the significant analysis about the Glucanase Exonuclease,the Glucanase enzyme and the total activity of three alternative strains,knew that those three activity of L-3 strain were 1701.7999 U,1880.0634 U,1798.2334 U,and it＇s significance was better than others ＇,so it was the best strains of efficient cellulase degradation.And this kind of strains is identified belonging to Penicillium. |
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| Keywords: | strain screening cellulase single-strain solid-state fermentation camellia cake |
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