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| 扭果花组织培养及快速繁殖的研究 | |
| 引用本文： | 陈雪娇,顾德峰,王刚,王海峰.扭果花组织培养及快速繁殖的研究[J].中国园艺文摘,2009,25(6):19-21. |
| 作者姓名： | 陈雪娇 顾德峰 王刚 王海峰 |
| 作者单位： | 1. 吉林农业大学园艺学院,吉林,长春,130118 2. 吉林省白河林业局两江林场,吉林白河,133600 3. 德惠市农业开发总公司,吉林,德惠,130300 |
| 摘 要： | 以扭果花试管苗叶片为外植体，MS为基本培养基，添加不同浓度的6-苄基腺嘌呤（6-BA）与萘乙酸（NAA），以此来探索这两种植物生长调节剂对扭果花叶片诱导不定芽的影响。结果表明，将外植体接种在MS＋BA0．5mg／L＋NAA0．5mg／L＋蔗糖30g／L＋琼脂9g／L上不定芽诱导率最高；不定芽继代增殖的最适培养基为MS＋BA2．0mg／L＋NAA0．2mg／L＋蔗糖30g／L＋琼脂9g／L；生根效果最佳培养基为MS＋NAA0．1mg／L＋蔗糖50g／L＋琼脂9g／L，生根率达100％。 |
| 关 键 词： | 扭果花 叶片 组织培养 快速繁殖 |
The Study on Tissue Culture and Rapid Propagation of Streptocarpus wendlanddii Spreng.ex Dammon |
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| Authors: | CHEN Xue-jiao GU De-feng WANG Gang WANG Hai-feng |
| Institution: | CHEN Xue-jiao GU De-feng WANG Gang WANG Hai-feng |
| Abstract: | In order to study the effects of two different plant growth regulators （cytokinin 6-BA and auxin NAA） on adventitious buds, the leaves of Streptocarpus wendlanddii Spreng.ex Dammon were used as explant for tissue culture, MS medium was the basic medium. The results showed that explant in MS ＋ BA0.Smg/L ＋ NAA0.5 mg/L＋ sucrose30g/ L＋agar9g/L can reach the highest rate of induced adventitious buds; MS ＋ BA2.0mg/L ＋ NAA0.2 mg/L＋ sucrose30g/ L＋agar9g/L was the most suitable one for the induction of adventitious buds. MS ＋ NAA0.1 mg/L＋ sucrose30g/L＋agar9g/ L was the most suitable one for the rooting,rooting rate reached 100%. |
| Keywords: | Streptocarpus wendlanddii Spreng ex dammon leaves tissue culture rapid propagation |
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