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三江白猪线粒体DNA-RFLP的分析
引用本文:杨隽,李馨,耿忠诚.三江白猪线粒体DNA-RFLP的分析[J].黑龙江畜牧兽医,2004(3):11-13.
作者姓名:杨隽  李馨  耿忠诚
作者单位:黑龙江八一农垦大学动物科技学院 黑龙江大庆163319 (杨隽,李馨),黑龙江八一农垦大学动物科技学院 黑龙江大庆163319(耿忠诚)
基金项目:黑龙江省农场总局“十五”科技攻关项目(HNK - 0 8- 0 1 )
摘    要:采用22种限制性内切酶分析了三江白猪线粒体DNA的多态性。结果表明:在60头个体中检出30种限制性态型.归结为7种基因单倍型;其间的差异来自少数限制性位点的突变。单倍型间平均遗传距离为0.35%,群体遗传多态程度为0.044%。三江白猪遗传多样性非常贫乏,说明三江白猪与地方种猪起源于一个共同祖先,在品种形成早期受到创立者效应的制约。

关 键 词:三江白猪  线粒体  DNA-RFLP  限制性内切酶  多态性  遗传  突变
文章编号:1004-7034(2004)03-0011-03
修稿时间:2003年7月18日

Analysis of the Polymorphism of Mitochondrial DNA in Sanjiang White Pig
YANG Jun,LI Xin,GENG Zhong-cheng.Analysis of the Polymorphism of Mitochondrial DNA in Sanjiang White Pig[J].Heilongjiang Animal Science And veterinary Medicine,2004(3):11-13.
Authors:YANG Jun  LI Xin  GENG Zhong-cheng
Abstract:A total of 22 restriction endonucleases were used to investigate the mitochondrial DNA polymorphism of Sanjiang White pigs. 30 restrictive morphs were detected, Which can be sorted into 7 haplotypes. The differences between haplotypes came from several dots mutation. The average genetic distance among haplotypes is 0.35%, and the value of average sequence variation in Sanjiang White pigs population is 0.044%.The results indicate that the genetic diversity based on restriction endomuclease cleavage patterns within Sanjiang White pigs is remarkably scarce. They may have originated from a late common ancestor, and were likely impacted by the founder effect.
Keywords:Sanjiang White pig  mtDNA  polymorphism  restriction endonucleases
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