| 猪轮状病毒的分离鉴定及部分特性研究 |
| |
| 引用本文： | 陈淑红,王新生,师东方,李一经.猪轮状病毒的分离鉴定及部分特性研究[J].中国预防兽医学报,2004,26(1):42-44. |
| |
| 作者姓名： | 陈淑红 王新生 师东方 李一经 |
| |
| 作者单位： | 东北农业大学,动物医学院,黑龙江,哈尔滨,150030 |
| |
| 摘 要： | 从吉林省某猪场发生腹泻的猪群粪便中,用MA-104细胞培养,分离到一株猪轮状病毒,盲传至4代,感染细胞出现明显病变,经电镜观察,见有典型的轮状病毒颗粒.其RNA电泳型为4:2:3:2型,属A群轮状病毒,同时对其蚀斑特性和血凝特性进行了初步研究,这株病毒被命名为JL94株.
|
| 关 键 词： | 猪 轮状病毒 分离鉴定 |
| 文章编号： | 1008-0589(2004)01-0042-03 |
| 修稿时间： | 2003年4月10日 |
Isolation and characterization of porcine rotavirus |
| |
| CHEN Shu-hong,WANG Xin-sheng,SHI Dong-fang,LI Yi-jing.Isolation and characterization of porcine rotavirus[J].Chinese Journal of Preventive Veterinary Medicine,2004,26(1):42-44. |
| |
| Authors: | CHEN Shu-hong WANG Xin-sheng SHI Dong-fang LI Yi-jing |
| |
| Abstract: | Porcine rotavirus was successfully isolated from diarrheal piglet fecal samples from Jilin Province. MA104 cells were used for the replication and isolation of the virus. After four generations , the strain could produce obvious cytopathic effects (CPE). Typical rotavirus particles were seen with direct electron microscope. Its RNA electropherotype was 4:2:3:2 which accords with the electropherotype of group A rotavirus. Its characteristics of hemagglutination and plague were studied tentatively. The strain of rotavirus was designated JL94. |
| |
| Keywords: | porcine rotavirus isolation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
