| 酶联免疫印迹对弓形虫抗原组分的研究 |
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| 引用本文： | 朱家勇.酶联免疫印迹对弓形虫抗原组分的研究[J].湛江医学院学报,1995,13(3):198-200. |
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| 作者姓名： | 朱家勇 |
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| 作者单位： | 广东医学院寄生虫学教研室 |
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| 摘 要： | 用酶联免疫印迹对弓形虫抗原组分分析的结果显示：经ＳＤＳ－ＰＡＧＥ后的弓形虫抗原组分分别为１．７８ＫＤ，２１．３ＫＤ，３０ＫＤ，３６．５ＫＤ，３９．１ＫＤ和５０．２ＫＤ。电转印至硝酸纤维膜上的各抗原组分，用ＥＬＩＢ分析，弓形虫感染宿主的血清能与抗原组分蛋白起反应，其中３９．１ＫＤ和２１．３ＫＤ分子量组分蛋白带色显色最深，提示这二种组分蛋白是弓形虫ＩｇＧ抗体识别的主要组分蛋白，而对照血清与各组分蛋白为
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| 关 键 词： | 诊断 抗原 弓形体病 酶联免疫印迹 |
IMMUNOBLOT ANALYSIS OF THE ANTIGEN OF TOXOPLASMA GONDII RECOGNIZED BY INFECTED RABBITS IgG ANTIBODIES |
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| Zhu Jiayong.IMMUNOBLOT ANALYSIS OF THE ANTIGEN OF TOXOPLASMA GONDII RECOGNIZED BY INFECTED RABBITS IgG ANTIBODIES[J].Journal of Guangdong Medical College,1995,13(3):198-200. |
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| Authors: | Zhu Jiayong |
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| Abstract: | Antigenic components of toxoplasma were determined by enzyme-linked immunoblotting technique(ELIB).The results showed that antigen protein bands were distributed under 50.2KD fields and had six bands that molecular weight were 17.8KD,21.3KD,30.0KD,36.5KD,39.1KD and 50.2KD respectively.The array of resolved antigen protein were electrophoretically transferred nitrocellulose filter paper and incubated with serum sample from rabbits with Toxoplasma gondii infections or with serum sample from seronegative controls,Serum immunoglobulin G bound to individual toxoplasma antigen were detected by HRP-stsphylococcal protein A.A complex array of toxoplasma antigens was revealed by this procedure.Immunoglobulin G directed against a low-molecular-weight antigen(two major antigens with apparent m.w.of 39100 and 21300)was detected in sera from rabbits infected with toxoplasma(10 sera tested)and absent from sera from individuals with seronegative controls(10 healthy rabbits,10 healthy persons,8 patients with entamoeba abscess of liver,10 rabbits infected with Schistosoma japonicum,3 patients with Plasmodium vivax infection). |
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| Keywords: | Toxoplasmosis/diagnostic use antigen/analysis SDS-PAGE ELIB serodiagnosis |
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