| Wolbachia在短管赤眼蜂和拟澳洲赤眼蜂种间的水平传递 |
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| 引用本文： | 潘雪红,何余容.Wolbachia在短管赤眼蜂和拟澳洲赤眼蜂种间的水平传递[J].西北农业学报,2011,20(4):186-188. |
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| 作者姓名： | 潘雪红 何余容 |
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| 作者单位： | 1. 广西甘蔗研究所,南宁530007;华南农业大学昆虫生态室,广州510640
2. 华南农业大学昆虫生态室,广州,510640 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 用米蛾卵作为短管赤眼蜂和拟澳洲赤眼蜂共寄生的寄主,实现Wolbachia在赤眼蜂种间的水平传递.对Wolbachia的wsp基因进行PCR检测,结果表明,这4头处女雌蜂体内有Wolbachia的存在,且在由这4头处女雌蜂为单系所建立第1～5代拟澳洲赤眼蜂群体内,Wolbaccha的检测均为阳性;wsp基因序列比对和同源性分析结果表明,拟澳洲赤眼蜂和短管赤眼蜂体内Wolbachia的wsp基因序列完全一致.证明未感染Wolbachia的拟澳洲赤眼蜂和感染Wolbachia的短管赤眼蜂在同一米蛾卵体内共同发育时,Wolbachia能从供体短管赤眼蜂成功地水平传递到新宿主拟澳洲赤眼蜂体内,并能在新宿主体内垂直传递5代.
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| 关 键 词： | 赤眼蜂 沃尔巴克氏体 产雌孤雌生殖 水平传递 |
Horizontal Transfer of Wolbachia between Trichogramma pretiosum and T. confusum |
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| PAN Xuehong and HE Yurong.Horizontal Transfer of Wolbachia between Trichogramma pretiosum and T. confusum [J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(4):186-188. |
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| Authors: | PAN Xuehong and HE Yurong |
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| Institution: | PAN Xuehong1,2 and HE Yurong2(1.Guangxi Sugarcane Research Institute,Nanning 530007,China,2.Lab.of Insect Ecology,South China Agricultural University,Guangzhou 510640,China) |
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| Abstract: | In this paper,eggs of Corcyra cephalonica Stainton were used as host eggs for synparasitism of Wolbachia-doner T.pretiosum and Wolbachia-recipient T.confusum.Interspecific horizontal transfer of Wolbachia between Trichogramma wasps was proved successfully.Wolbachia was detected by PCR of wsp gene of Wolbachia for up to 5 generations after transferring.The wsp sequence of Wolbachia in new hosts T.confusum was identical to that of T.pretiosum.Therefore,Wolbachia from an infected species,T.pretiosum,were succe... |
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| Keywords: | Trichogramma Wolbachia Thelytokous parthenogenesis Horizontal transfer |
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