| 水稻抗稻瘟病基因Pi-ta的分子标记辅助选择 |
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| 引用本文： | 王忠华,贾育林,吴殿星,夏英武.水稻抗稻瘟病基因Pi-ta的分子标记辅助选择[J].作物学报,2004,30(12):1259-1265. |
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| 作者姓名： | 王忠华 贾育林 吴殿星 夏英武 |
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| 作者单位： | 浙江万里学院生物技术研究所, 浙江宁波315100 |
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| 基金项目： | 美国阿肯色州水稻研究和发展基金，浙江省高等学校青年教师资助计划 |
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| 摘 要： | 利用已建立的水稻抗稻瘟病基因Pi-ta显性分子标记对30个品系和157个来自不同国家的一些水稻品种进行分子鉴定，并采用稻瘟病菌菌株ZN57（IC-17）和ZN61（IB-49）人工接种试验进行致病性测试。结果表明，大部分品系和少数水稻品种含抗病基因Pi-ta，且对稻瘟病菌菌株ZN57和ZN61表现抗病反应。除此之外，利用两对显性分子标记YL1
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| 关 键 词： | 水稻 抗稻瘟病基因Pi-ta 分子标记 辅助选择 |
| 收稿时间： | 2003-06-30 |
| 修稿时间： | 2003年6月30日 |
Molecular Markers-assisted Selection of the Rice Blast Resistance Gene Pi-ta |
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| WANG Zhong Hua ,,JIA Yu Lin ,WU Dian Xing ,XIA Ying Wu.Molecular Markers-assisted Selection of the Rice Blast Resistance Gene Pi-ta[J].Acta Agronomica Sinica,2004,30(12):1259-1265. |
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| Authors: | WANG Zhong Hua JIA Yu Lin WU Dian Xing XIA Ying Wu |
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| Institution: | Institute of Biotechnology, Zhejiang Wanli University, Ningbo 315100, Zhejiang |
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| Abstract: | The dominant molecular markers of the rice blast resistance gene Pi ta were used to identify the presence in the Pi ta gene of 30 breeding lines and 157 rice cultivars coming from some different countries Meanwhile pathogenicity assays were performed with the rice blast fungus strains ZN57(IC 17) and ZN61(IB 49) The results indicated that most breeding lines and several rice cultivars contained the Pi ta gene and were resistant to strains ZN57 and ZN61 In addition,two pairs of dominant markers YL155/YL87 and YL183/YL87 were used to screen the resistant breeding lines at the early stage One hundred and eighteen lines containing homozygous Pi ta gene were isolated from 350 F 3 breeding lines At the same time,the rice blast resistances of these lines in the field were investigated The results demonstrated that the Pi ta presence consisted with the resistance in the field Considering that Pi ta is closely linked with other blast resistance genes,the Pi ta gene markers is very useful for marker assisted selection |
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| Keywords: | Rice( Oryza sativa L ) Rice blast resistance Pi ta gene Molecular markers Marker assisted selection |
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