| 肉仔鸡胸肌卫星细胞氧化应激模型的优化 |
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| 引用本文： | 王成,武书庚,岳洪源,翟永功,张海军,齐广海.肉仔鸡胸肌卫星细胞氧化应激模型的优化[J].家畜生态,2010(2):29-34. |
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| 作者姓名： | 王成 武书庚 岳洪源 翟永功 张海军 齐广海 |
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| 作者单位： | 中国农业科学院饲料研究所;北京师范大学生命科学学院; |
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| 基金项目： | 国家科技支撑计划(2006BAD12B06);;云南省科技创新强省计划(2007AD003) |
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| 摘 要： | 将体外培养的肉仔鸡胸肌卫星细胞（SC）按地塞米松（DEX）处理浓度（0、0.03、0.06、0.12、0.24、0.48和0.96 mg/mL）分为7组,分别测定SC的存活率（MTT法）、丙二醛（MDA）、氧自由基（ROS）、超氧化物歧化酶（SOD）和谷胱甘肽硫转酶（GST）含量或活性。筛选DEX处理的最佳时间和浓度以便优化肉仔鸡胸肌SC的氧化应激模型。结果表明,随DEX浓度升高,MDA和ROS产生量升高（P〈0.01）,SOD与GST活性显著降低（P〈0.01）。随着DEX处理时间的延长,SC的存活率及GST和SOD活性均有所下降,MDA和ROS产生量上升。使用0.12 mg/mL的DEX处理SC 24 h后,SC存活率显著降低（P〈0.01）。说明DEX浓度为12 mg/mL处理24 h能使肉仔鸡骨骼肌SC产生适度的氧化应激,可以作为肉仔鸡胸肌卫星细胞的氧化应激模型。
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| 关 键 词： | 地塞米松 肉仔鸡 胸肌卫星细胞 氧化应激模型 |
Optimization of Oxidative Stress Model From Broiler Breast Muscle Satellite Cells |
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| WANG Cheng,WU Shu-geng,YUE Hong-yuan,ZHAI Yong-gong,ZHANG Hai-jun,QI Guang-hai.Optimization of Oxidative Stress Model From Broiler Breast Muscle Satellite Cells[J].Ecology of Domestic Animal,2010(2):29-34. |
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| Authors: | WANG Cheng WU Shu-geng YUE Hong-yuan ZHAI Yong-gong ZHANG Hai-jun QI Guang-hai |
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| Institution: | 1.Feed Research Institute;Chinese Academy of Agricultural Sciences;Beijing 100081;China;2.College of Life Sciences;Beijing Normal University;Beijing 100875;China |
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| Abstract: | Time and concentration of dexamethasone phosphate solution(DEX) were optimized to establish oxidative stress model of boiler breast muscle satellite cells(SC).Seven concentrations of DEX(0,0.03,0.06,0.12,0.24,0.48 and 0.96 mg/mL) were used to treat boiler breast muscle SC,Methyl thiazolyl tetrazolium(MTT)assay was used to assess cell livability.The relative production of malonaldehyde(MDA),reactive oxidative species(ROS),the activities of superoxide dismutase(SOD) and glutathione S-transferase(GST) were mea... |
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| Keywords: | dexamethasone phosphate solution boiler breast satellite cell oxidative stress model |
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