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| 小麦Ta-hir2基因克隆及转Ta-hir2基因小麦的获得 | |
| 引用本文： | 陈佳平,孟涛,刘芬,于秀梅,赵伟全,刘大群.小麦Ta-hir2基因克隆及转Ta-hir2基因小麦的获得[J].河北农业大学学报,2011,34(5):8-13. |
| 作者姓名： | 陈佳平 孟涛 刘芬 于秀梅 赵伟全 刘大群 |
| 作者单位： | 1. 河北农业大学生命科学学院,河北保定,071001 2. 河北农业大学生命科学学院,河北保定071001;河北农业大学植物保护学院,河北保定071001 3. 河北农业大学植物保护学院,河北保定071001;河北省农作物病虫害生物防治工程技术研究中心,河北保定071001 |
| 基金项目： | 河北省自然科学基金，国家自然科学基金 |
| 摘 要： | 通过基因枪介导法将小麦过敏性诱导反应蛋白2基因Ta-hir2转入小麦胚性愈伤组织中,经分化筛选后获得转基因小麦植株,为验证该基因的功能奠定基础.以接种非亲和叶锈菌小种05-19-43②的小麦‘TcLr15,为材料,采用同源克隆的策略克隆了小麦Ta-hir2基因；将该基因的编码区与植物表达载体pAHC-25相连,成功构建... |
| 关 键 词： | 小麦 过敏性诱导反应蛋白2 基因克隆 载体构建 |
Cloning of a wheat gene Ta-hir2 and obtaining of transgenic plant of wheat with Ta-hir2 |
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| CHEN Jia-ping,MENG Tao,LIU Fen,YU Xiu-mei,ZHAO Wei-quan,LIU Da-qun.Cloning of a wheat gene Ta-hir2 and obtaining of transgenic plant of wheat with Ta-hir2[J].Journal of Agricultural University of Hebei,2011,34(5):8-13. | |
| Authors: | CHEN Jia-ping MENG Tao LIU Fen YU Xiu-mei ZHAO Wei-quan LIU Da-qun |
| Institution: | 2,3(1.College of Life Science,Agricultural University of Hebei,Baoding 071001,China;2.College of Plant Protection,Agricultural University of Hebei,Baoding 071001,China;3.Biological Control Centre of Plant Diseases and Plant Pests of Hebei Province,Baoding 071001,China) |
| Abstract: | The purpose of the present study was to obtain transgenic plant of wheat through transforming wheat hypersensitive induced reaction protein 2 gene Ta-hir2 into wheat embryogenic callus by particle bombardment,and series of differentiation and selection,which would lay a foundation for determining the function of Ta-hir2 in wheat.Ta-hir2 was cloned from wheat(Triticum aestivum L.) isogenic line ’TcLr15’ inoculated with incompatible leaf rust race 05-19-43② by homology cloning.The recombinant plasmid Ta-hir2-pAHC-25 was constructed successfully by inserting open reading frame of Ta-hir2 into the plant expression vector pAHC-25.Embryogenic callus initiated from mature embryos of ’Thatcher’ and ’TcLr15’ were transformed with recombinant Ta-hir2-pAHC-25 by particle bombardment,and the regenerated seedlings were obtained.Genomic DNA of regenerated seedlings were extracted and subject to PCR detection,the positive ratio for PCR was 4.76%,ratio of gene transformation was 0.28%. |
| Keywords: | wheat hypersensitive induced reaction protein 2 gene cloning vector construction |
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