| 基于无毒基因的晚疫病菌指纹类型与致病型关系研究 |
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| 引用本文： | 李本金,陈昌盛,兰成忠,黄灿强,翁启勇,陈庆河.基于无毒基因的晚疫病菌指纹类型与致病型关系研究[J].植物病理学报,2011,41(3):328-332. |
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| 作者姓名： | 李本金 陈昌盛 兰成忠 黄灿强 翁启勇 陈庆河 |
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| 作者单位： | 福建省农业科学院植物保护研究所,福州,350013 |
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| 基金项目： | 国家自然科学基金资助项目，福建省自然科学基金资助项目 |
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| 摘 要： | 由致病疫霉Phytophthora infestans(Mont)de Bary引起的晚疫病是马铃薯生产上可导致绝收的世界性病害,在我国各地均有发生和流行1].传统的致病型鉴别只局限于表型上的分析,无法对复杂多变的晚疫病菌致病型变化进行快速准确的预测,因此有必要探索新的方法来弥补常规手段的不足,以便更加准确有效地监测其变化趋势.晚疫病菌与寄主马铃薯这一病理体系中的单一抗性基因和相应的无毒基因符合典型"基因对基因"学说,因此基于抗病基因与无毒基因的关系对晚疫病菌分类将更为科学和实用.到目前为止,已知的晚疫病菌无毒基因序列有Avr1、Avr2、Avr3a和Avr42,3].本研究试图分析晚疫病菌基于无毒基因的指纹类型与马铃薯鉴别品种划分的致病型之间是否存在对应关系,以期进一步了解病原菌群体结构特征及遗传变异本质,从而更好地指导抗晚疫病育种和品种的合理布局.
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| 关 键 词： | 指纹 抗性基因 致病型 晚疫病菌 马铃薯 菌株 无毒基因 抗病基因 特异引物 |
| 收稿时间： | 2010-08-30; |
Correlation between pathotype and DNA fingerprinting pattern based on avirulence genes of Phytophthora infestans |
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| LI Ben-jin,CHEN Chang-sheng,LAN Cheng-zhong,HUANG Can-qiang,WENG Qi-yong,CHEN Qing-he.Correlation between pathotype and DNA fingerprinting pattern based on avirulence genes of Phytophthora infestans[J].Acta Phytopathologica Sinica,2011,41(3):328-332. |
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| Authors: | LI Ben-jin CHEN Chang-sheng LAN Cheng-zhong HUANG Can-qiang WENG Qi-yong CHEN Qing-he |
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| Institution: | LI Ben-jin,CHEN Chang-sheng,LAN Cheng-zhong,HUANG Can-qiang,WENG Qi-yong,CHEN Qing-he(Institute of Plant Protection,Fujian Academy of Agricultu-ral Sciences,Fuzhou 350013,China) |
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| Abstract: | Four specific primers were designed according to the sequences of avirulence genes of Phytophthora infestans in GenBank and 88 P.infestans isolates from various regions were used for amplification by PCR technique with the primers.Seven types of DNA fingerprinting were obtained by assortment of the bands of PCR products.Meanwhile,pathotypes were identified with 11 different potato cultivars carrying known dominant mono-genes R1-R11 according to the response of resistance and susceptibility,the results showe... |
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| Keywords: | Phytophthora infestans avirulence gene DNA fingerprinting pattern differential cultivar pathotype |
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