| 乳胶凝集试验检测牛羊淋巴结中的沙门氏菌 |
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| 引用本文： | 申什菊,王林业.乳胶凝集试验检测牛羊淋巴结中的沙门氏菌[J].现代畜牧兽医,2008(9). |
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| 作者姓名： | 申什菊 王林业 |
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| 作者单位： | 1. 青海省海东地区互助县高寨镇兽医站,青海,互助,810500
2. 青海省海东地区互助县塘川镇畜牧兽医服务中心,青海,互助,810500 |
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| 摘 要： | 采用沙门氏菌多价血清致敏乳胶并制成乳胶抗体,建立沙门氏菌乳胶凝集试验检测方法(LAT),分别用LAT法和常规分离培养检测法对38份牛淋巴结、101份羊淋巴结进行检测.结果:乳胶凝集试验牛淋巴结阳性21份,羊淋巴结阳性44份;常规分离培养检测法牛淋巴结阳性22份,羊淋巴结阳性48份,两种方法的阳性符合率均超过80%.试验表明乳胶凝集试验操作简便、快速、敏感性高、特异性强且可用于现场检测,是一种适合基层单位检测沙门氏菌的可靠方法.
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| 关 键 词： | 乳胶凝集试验 检测 沙门氏菌 牛羊淋巴结 |
Latex Agglutination Test to Detect Salmonella in the Cattle-Sheep Lymph Nodes |
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| Shen Shiju,Wang Linye.Latex Agglutination Test to Detect Salmonella in the Cattle-Sheep Lymph Nodes[J].Modern JOurnal of Animal Husbandry and Veterinary Medicine,2008(9). |
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| Authors: | Shen Shiju Wang Linye |
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| Abstract: | Salmonella multipartial serum was used to sensitize latex treated formerly and the latex antibodies was prepared. The latex agglutinative test (LAT) to detect salmonella was established.38 cattle lymph nodes,101 sheep lymph nodes were tested by LAT and technology of routine isolated culture.Positive number is 21 in cattle lymph nodes and 44 in sheep lymph nodes by LAT .Positive number is 22 in cattle lymph nodes and 48 in sheep lymph nodes by routine method.The coincidence rate of both methods reached 83.3% in manuforage,81.8% in cattle lymph nodes and 81.3% in sheep lymph nodes respectively. The results showed that LAT has advantages such as simply,rapid,sensitive,specifical and so on.It is suitable for grass-roots unit to detect salmonella. |
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| Keywords: | Latex agglutinative test Detect Salmonella Cattle-sheep lymph nodes |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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