A SCAR marker specific for rapid detection of the avirulence gene Avr1c in Phytophthora sojae |
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| Authors: | Jingzhi Wen Qiuming Chen Long Sun Liming Zhao Bing Suo Miao Tian |
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| Institution: | 1. Department of Plant Protection, College of Agriculture, Northeast Agricultural University, Harbin, 150030, People’s Republic of China
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| Abstract: | The F2 population derived from a cross between isolates pRx (Avr1c-Avr1c) and ps1 (avr1c-avr1c) of Phytophthora sojae, fungal agent of soybean stem and root rot, was used to determine the genetic basis of avirulence towards Rps1c gene in soybean. The results indicated that this avirulence is dominant and controlled by a single locus, as expected for a simple gene-for-gene model. Segregation of Avr1c in the F2 progeny of this cross fits a 3:1 ratio. Four of 80 AFLP primers effectively distinguished the avirulent pRx from the virulent ps1. Among the 5 specific markers, band C was amplified from the avirulent pRx by primer set EGC/MAT, then recovered and cloned. This AFLP marker was successfully transfered to a SCAR marker through sequencing, primer design and specific amplication of the DNA of the avirulent pRx. Results of validity and specificity experiments with 50 individuals of the F2 progeny and 50 field isolates demonstrated that this SCAR marker (a 616-bp fragment) can be successfully and specifically amplified from the P. sojae isolates that have Avr1c gene. |
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