| 羊附红细胞体PCR诊断方法的建立及应用 |
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| 引用本文： | 周作勇,聂奎,周荣琼,刘凤英,陈艳灵.羊附红细胞体PCR诊断方法的建立及应用[J].西南农业大学学报,2010,32(2). |
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| 作者姓名： | 周作勇 聂奎 周荣琼 刘凤英 陈艳灵 |
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| 作者单位： | 西南大学荣昌校区动物医学系;西南大学动物科技学院; |
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| 基金项目： | 重庆市自然科学基金资助项目(CSTC,2006BA1009) |
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| 摘 要： | 根据羊附红细胞体16S rRNA基因序列设计特异性引物,建立了羊附红细胞体的PCR诊断方法.该方法能扩增羊附红细胞体16S rRNA基因片段,对大肠杆菌、羊无形体、羊源支原体、猪附红体和假单孢菌基因组DNA没有扩增出条带,所能检测羊附红细胞体DNA的最低量为1.82 pg/mL.运用所建立的方法对从重庆市荣昌县采集的110份羊血样进行附红细胞体检测,其中13份羊附红体感染阳性,表明该方法特异性和灵敏度高,可用于急性羊附红细胞体病和临床带菌羊的检测.
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| 关 键 词： | 羊附红细胞体 PCR 检测 |
Development and Preliminary Application of PCR Assay Based on the 16S rRNA Gene for Detection of Eperythrozoon ovis in Sheep |
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| ZHOU Zuo-yong,NIE Kui,ZHOU Rong-qiong,LIU Feng-ying,CHEN Yan-ling.Development and Preliminary Application of PCR Assay Based on the 16S rRNA Gene for Detection of Eperythrozoon ovis in Sheep[J].Journal of Southwest Agricultural University,2010,32(2). |
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| Authors: | ZHOU Zuo-yong NIE Kui ZHOU Rong-qiong LIU Feng-ying CHEN Yan-ling |
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| Institution: | ZHOU Zuo-yong1,NIE Kui2,ZHOU Rong-qiong1,LIU Feng-ying1,CHEN Yan-ling11.Department of Veterinary Medicine,Rongchang Campus,Southwest University,Rongchang Chongqing 402460,China,2.School of Animal Science , Technology,Chongqing 400716 |
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| Abstract: | Based on the sequence data of 16S rRNA gene of Eperythrozoon ovis,a set of E.ovis specific primers were designed.No PCR products were amplified from purified DNA of Escherichia coli,Anaplasma ovis,Mycoplasma ovis,Eperythrozoon suis and Pseudomonas putid.It was estimated that as few as 1.82 pg/mL E.ovis genomic DNA was detectable by PCR.Using this method,110 blood samples from Rongchang were successfully detected and 13 samples were positive with E.ovis infection.The results showed the PCR method developed w... |
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| Keywords: | Eperythrozoon ovis PCR detection |
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