| 不同细胞密度冻存对奶牛乳腺上皮细胞的影响 |
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| 引用本文： | 杨银芬,考桂兰,侯先志,高爱武,韩亚楠,赵青.不同细胞密度冻存对奶牛乳腺上皮细胞的影响[J].畜牧与饲料科学,2011(9). |
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| 作者姓名： | 杨银芬 考桂兰 侯先志 高爱武 韩亚楠 赵青 |
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| 作者单位： | 内蒙古农业大学动物科学学院,内蒙古呼和浩特,010018 |
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| 基金项目： | 农业部“奶牛产业技术体系”项目子课题 |
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| 摘 要： | 试验采用了内蒙古呼和浩特健康荷斯坦奶牛乳腺组织经5代纯化后,将奶牛乳腺上皮细胞分别设为1组（1×104个细胞/mL）、2组（1×105个细胞/mL）、3组（1×106个细胞/mL）、4组（1×107个细胞/mL）4个密度梯度进行冻存,复苏后检测细胞死亡率、贴壁率、凋亡率并进行形态学观察。结果显示,4组复苏后死亡率极显著低于其他3组（P〈0.01）,贴壁率显著高于其他3组（P〈0.05）;3组细胞凋亡率显著低于其他3组（P〈0.05）;3、4组细胞到第3天基本铺满瓶底,生长状态良好。
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| 关 键 词： | 奶牛乳腺上皮细胞 死亡率 贴壁率 凋亡率 |
Effect of Different Cells Density on the Cryopreservation of Bovine Mammary EpithelialCells |
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| Abstract: | In this study,the breast tissue of a healthy Holstein dairy cow was used.Through five-generation purification,the bovine mammary epithelial cells were divided into four groups,according to the cell density（1×104 cells/mL,group 1;1×105 cells/mL,group 2;1×106 cells/mL,group 3;1×107 cells/mL,group 4, respectively）and cryopreservated. One month later the cryopreservated cells were recovered and cell viability was measured.The results showed that the death rate of bovine mammary epithelial cell was significantly lower for group 4 than that of other treatments（P0.01）, whereas the reverse result was found for cell adhesion rate among four treatments （P0.05）.As compared with other groups,group 3 had the lowest apoptosis rate（P0.05）.The cells of group 3 and group 4 basically covered with the bottom of the culture flask and the cells were in good condition at three days after adherence. |
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| Keywords: | bovine mammary epithelial cells death rate adhesion rate apoptosis rate |
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