| 宁夏稻瘟病菌的交配型与育性 |
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| 引用本文： | 李文强,王源超,郑小波.宁夏稻瘟病菌的交配型与育性[J].中国水稻科学,2007,21(6):650-656. |
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| 作者姓名： | 李文强 王源超 郑小波 |
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| 作者单位： | 1. 南京农业大学,植物保护学院/农业部病虫监测与治理重点开放实验室,江苏,南京,210095;宁夏大学,农学院,宁夏,银川,750021
2. 南京农业大学,植物保护学院/农业部病虫监测与治理重点开放实验室,江苏,南京,210095 |
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| 基金项目： | 国家高技术研究发展计划(863计划);国家科技支撑计划 |
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| 摘 要： | 用4个高育性标准菌株对2002-2005年在宁夏9个种植水稻的市县采集的460个稻瘟病菌单孢菌株的交配型和育性进行了测定。在460个菌株中有363个（78.9％）能与4个标准菌株之一杂交产生子囊壳，其余97个（21.1％）菌株不能与4个标准菌株中的任何一个杂交产生子囊壳，是不育菌株。所有363个可育菌株都是交配型MAT1 1的雄性菌株，没有发现交配型是MAT1 2的菌株，也没有发现雌性可育菌株和两性可育菌株。在363个可育菌株中，有79个(21.8%)产生子囊壳、子囊和子囊孢子，其余菌株产生的子囊壳中没有子囊孢子。地理位置、年份和水稻品种对宁夏稻瘟病菌群体的交配型分布和育性没有影响。用PCR技术对宁夏的稻瘟病菌的交配型进行了快速检测，所有460个菌株都属于交配型MAT1 1。这一结果与标准菌株检测的结果一致。只存在一种交配型和缺乏雌性可育菌株表明宁夏稻瘟病菌群体没有有性生殖发生。
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| 关 键 词： | 稻瘟病菌 有性态 育性 交配型 |
| 文章编号： | 1001-7216（2007）06-0650-07 |
| 收稿时间： | 2007-03-20 |
| 修稿时间： | 2007-05-23 |
Mating Type and Fertility of Magnaporthe grisea Populations from Rice in Ningxia Hui Autonomous Region, China |
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| LI Wen-qiang,WANG Yuan-chao,ZHENG Xiao-bo.Mating Type and Fertility of Magnaporthe grisea Populations from Rice in Ningxia Hui Autonomous Region, China[J].Chinese Journal of Rice Science,2007,21(6):650-656. |
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| Authors: | LI Wen-qiang WANG Yuan-chao ZHENG Xiao-bo |
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| Institution: | 1 Key Laboratory of Monitoring and Management of Plant Disease and Insects of the Ministry of Agriculture/College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China ;2 College of Agronomy, Ningxia University, Yinchuan 750021 , China |
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| Abstract: | To determine mating type and fertility of Magnaporthe grisea populations from rice in Ningxia Hui Autonomous Region,northwestern China,four hundred and sixty isolates from different rice varieties were tested with high fertile standard testers including TH12(MAT1-1),KA3(MAT1-1),TH16(MAT1-2),and Guy11(MAT1-2)in 2002-2005.Of the 460 monoconidial isolates,363(78.9%)were fertile and produced perithecia when paired with either of the four testers,and 97(21.1%)were infertile and did not produce perithecia when mated with any of the four testers.All fertile isolates were male fertile and belonged to a single mating type,MAT1-1,no female fertile and hermaphroditic isolates were detected in sample populations.Among the 363 fertile isolates,79(21.8%)produced perithecia,asci,and ascospores,others produced only barren perithecia.Mating type distribution and fertility status were irrespective of the host variety or geographical location or year of collection of isolates.In a PCR-based mating type assay,the total 460 M.grisea field isolates were identified as MAT1-1,which entirely corroborated the result of standard mating assays using fertile testers.The presence of only one mating type and the absence of female fertile isolates indicated that sexual reproduction was rare or absent in M.grisea populations associated with rice in Ningxia Hui Autonomous Region,northwestern China. |
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| Keywords: | Magnaporthe grisea perfect stage fertility mating type rice |
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