| 常春藤离体快繁技术 |
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| 引用本文： | 张存旭,杨锋利,袁秀平.常春藤离体快繁技术[J].浙江林学院学报,2005,22(2):241-245. |
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| 作者姓名： | 张存旭 杨锋利 袁秀平 |
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| 作者单位： | 1. 西北农林科技大学,林学院,陕西,杨凌,712100
2. 杨凌职业技术学院,林学系,陕西,杨凌,712100 |
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| 摘 要： | 以常春藤Hedera nepalensis var.sinensis茎段为外植体,研究了培养基、植物生长调节物质和移栽基质对常春藤离体快繁的影响.结果表明:起始和增殖培养中高盐质量浓度的MS培养基好于低盐培养基WPM和B5.增殖培养时,在培养基中同时添加2.0 mg·L-16-BA和1.0 mg·L-1GA3为佳,增殖系数可达到4.以1/2 MS为基本培养基,虽然不添加任何生长调节物质亦能获得高生根率,但附加0.10 mg·L-1NAA能更好地促进根系发育.移栽基质选用腐质土,成活率可达80%以上.用自来水代替蒸馏水,白砂糖代替蔗糖,对常春藤试管苗的增殖和生长无显著影响,可降低培养成本.表6参9
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| 关 键 词： | 育苗 常春藤 快速繁殖 体外培养 |
| 文章编号： | 1000-5692(2005)02-0241-05 |
In vitro rapid propagation of Hedera nepalensis var.sinensis |
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| ZHANG Cun-xu,YANG Feng-li,YUAN Xiu-ping.In vitro rapid propagation of Hedera nepalensis var.sinensis[J].Journal of Zhejiang Forestry College,2005,22(2):241-245. |
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| Authors: | ZHANG Cun-xu YANG Feng-li YUAN Xiu-ping |
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| Abstract: | Using nodal segments as a source of initial explants,the effects of basal medium,plant growth substances as well as transplantation medium on rapid propagation of Hedera neplensis var.sinensis was studied.The results showed that the rich salt medium MS is more suitable for initial and multiplication culture than low salt media WPM and B_5.It is better to add 2.0 mgL~(-1)6-BA and 1.0 mgL~(-1)GA_3 to multiplication medium,in which multiplication coefficient reached 4.Although high rooting percentage was obtained with free growth regulators on 1/2 MS medium,root system development could be improved on medium by adding 0.10 mgL~(-1) NAA.The survival rate of transplanation was about 80% in humus soil.Using tap water and edible white sugar instead of distilled water and sucrose have no significant effect on shoots multiplication and growth of H. nepalensis var.sinensis,so the culture cost could be reduced.Ch,6 tab.9 ref.] |
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| Keywords: | nursery stock growing Hedera nepalensis var sinensis rapid propagation in vitro culture |
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