| 北柴胡新品种川北柴1号组织培养快繁初步研究 |
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| 引用本文： | 余马,左文松,刘茹,徐冬梅,舒晓燕,唐志康,侯大斌.北柴胡新品种川北柴1号组织培养快繁初步研究[J].种子,2017(9). |
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| 作者姓名： | 余马 左文松 刘茹 徐冬梅 舒晓燕 唐志康 侯大斌 |
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| 作者单位： | 1. 西南科技大学生命科学与工程学院,四川绵阳,621010;2. 泸州市古蔺县农业局,四川泸州,646500 |
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| 基金项目： | 国家自然科学基金(81603223)，四川省“十三五”科技育种攻关(2016 NYZ 0036-4-2)，四川省科技扶贫专项(2016 FP0071 |
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| 摘 要： | 目的]研究适宜于北柴胡新品种川北柴1号的组织培养快繁技术.方法]以川北柴1号茎节为材料,分别以MS和1/2 MS为基本培养基,通过不同的激素种类和浓度,分析诱导川北柴1号丛生芽和不定根生长的最佳培养基配方.结果]在MS培养基中,1.0 mg/L的6-BA,0.5 mg/L的KT,0.2 mg/L的NAA和1.0 mg/L的IAA在显著促进不定芽增殖的同时,还能最大化促进不定芽生长;在1/2 MS培养基中,0.1 mg/L浓度NAA,1.0mg/L浓度IBA和1.0 mg/L浓度IAA对柴胡不定根生长促进作用最佳.结论]本研究对川北柴1号生产推广制种具有重大意义.
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| 关 键 词： | 北柴胡 川北柴1号 生长激素 组织培养 |
Plant Regeneration Through Tissue Culture for Commercial Variety of Chuanbeichai No.1 in Bupleurum chinense DC. |
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| YU Ma,ZUO Wensong,LIU Ru,XU Dongmei,SHU Xiaoyan,TANG Zhikang,HOU Dabin.Plant Regeneration Through Tissue Culture for Commercial Variety of Chuanbeichai No.1 in Bupleurum chinense DC.[J].Seed,2017(9). |
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| Authors: | YU Ma ZUO Wensong LIU Ru XU Dongmei SHU Xiaoyan TANG Zhikang HOU Dabin |
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| Abstract: | Objective]identify a protocol of tissue culture for commercial variety of Chuanbeichai No.1 in Bupleurum chinense DC.Method]the stem of Chuanbeichai No.1 was used to induce shoots in MS medium,and subsequent induce root in 1/2 MS medium.The effect of growth regulators with different concentrations was also analyzed.Results] the adding of 1.0 mg/L 6-BA,0.5 mg/L KT,0.2 mg/L NAA,or 1.0 mg/L IAA in MS medium resulted in the best multiplication.The adding of 0.1 mg/L NAA,1.0 mg/L IBA,or 1.0 mg/L IAA in 1/2 MS medium resulted in the best root formation.Conclusion] this micropropagation is very important to large scale of seed production for Chuanbeichai No.1. |
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| Keywords: | Bupleurum chinense DC chuanbeichai No 1 growth regulators tissue culture |
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