| 进境新西兰猕猴桃中猕猴桃果腐病菌的检测与鉴定 |
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| 引用本文： | 张露茜,刘静远,徐飞,焦彬彬,于翠,杨翠云.进境新西兰猕猴桃中猕猴桃果腐病菌的检测与鉴定[J].植物检疫,2017(4):46-49. |
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| 作者姓名： | 张露茜 刘静远 徐飞 焦彬彬 于翠 杨翠云 |
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| 作者单位： | 上海出入境检验检疫局 上海200135 |
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| 基金项目： | 上海出入境检验检疫局科技计划项目(HK001-2017) |
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| 摘 要： | 对来自新西兰的猕猴桃进行了病原菌的分离培养,结果在果实中分离到2株疑似猕猴桃果腐病菌的菌株Na-3419和Na-4756。对其进行了病原菌形态学观察、致病性测定并结合分子生物学方法检测,实验结果表明,菌株Na-3419和Na-4756在PDA培养基上生长良好,能产生大量的分生孢子;经真菌通用引物(ITS4/ITS5)扩增和测序,菌株Na-3419与Gen Bank中登录号EU482221.1和EU482220.1的菌株序列同源性达到100%,菌株Na-4756与Gen Bank中登录号AY359230.1、AY359226.1和KR859079.1的菌株序列同源性达到100%;病菌接种猕猴桃3 d后开始发病。根据上述试验结果,将分离获得的菌株Na-3419和Na-4756鉴定为猕猴桃果腐病菌(Neofabraea actinidiae)。这是上海口岸首次在进境的猕猴桃果实上截获该有害生物。
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| 关 键 词： | 猕猴桃果腐病菌 形态特征 分子检测 致病性测定 鉴定 |
Detection and identification of Neofabraea actinidiae on imported Actinidia chinensis from New Zealand |
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| Zhang Luxi,Liu Jingyuan,Xu Fei,Jiao Binbin,Yu Cui,Yang Cuiyun.Detection and identification of Neofabraea actinidiae on imported Actinidia chinensis from New Zealand[J].Plant Quarantine,2017(4):46-49. |
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| Authors: | Zhang Luxi Liu Jingyuan Xu Fei Jiao Binbin Yu Cui Yang Cuiyun |
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| Abstract: | The isolates Na-3419 and Na-4756 were isolated on imported Actinidia chinensis from New Zealand by traditional PDA method.They grew well on PDA,producing lots of conidia.The isolate DNA was amplificated and sequenced by universal primers.Compared with the identity of DNA sequence of Neofabraea actinidiae (Accession..AY359230.1,AY359226.1,KR859079.1,EU482221.1,EU482220.1) from NCBI GenBank databases by blast method,the result showed that its homology was up to 100％.Pathogenicity tests showed that kiwiffuit was infected by Na-3419 and Na-4756.Based on morphological characteristics,molecular detection and pathogenicity test results,the isolates Na-3419 and Na-4756 were identified as Neofabraea actinidiae.This pest was intercepted on imported Actinidia chinensis for the first time in Shanghai. |
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| Keywords: | Neofabraea actinidiae morphological characteristics molecular detection pathogenicity test identification |
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