| 无花果树苗中截获的无花果孢囊线虫形态和分子鉴定 |
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| 引用本文： | 宋绍祎,俞禄珍,戚龙君,于子翔.无花果树苗中截获的无花果孢囊线虫形态和分子鉴定[J].植物检疫,2017(4):38-42. |
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| 作者姓名： | 宋绍祎 俞禄珍 戚龙君 于子翔 |
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| 作者单位： | 上海出入境检验检疫局 上海200135 |
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| 基金项目： | 国家重点研发计划“生物安全关键技术研发”重点专项(2017YFC1200602)，上海出入境检验检疫局科研项目(HK003-2017 |
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| 摘 要： | 上海口岸从旅客携带进境的树苗中分离到1种孢囊线虫属的2龄幼虫,通过形态特征和分子特征分析,鉴定为无花果孢囊线虫(Heterodera fici Kirjanova,1954)。该2龄幼虫的主要形态特征为:体长409.9~454.4μm;唇环3个;口针发达,长22.6~24.0μm,基部球圆形略向前倾斜;侧线4条;尾长圆锥形,末端细圆,尾部透明区长24.7~30.5μm,约占尾长的1/2。序列分析显示其ITS区序列与GenBank中无花果孢囊线虫的序列相似性为99.48%~99.90%,序列差异为1~5 bp。系统发育分析显示该线虫与无花果孢囊线虫处于一个聚类组内。这是我国口岸首次报道截获无花果孢囊线虫。
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| 关 键 词： | 无花果孢囊线虫 形态特征 系统发育 鉴定 |
Morphological and molecular identification of Heterodera fici in fig tree seedlings |
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| Song Shaoyi,Yu Luzhen,Qi Longjun,Yu Zixiang.Morphological and molecular identification of Heterodera fici in fig tree seedlings[J].Plant Quarantine,2017(4):38-42. |
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| Authors: | Song Shaoyi Yu Luzhen Qi Longjun Yu Zixiang |
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| Abstract: | The second-stage juveniles (J2s) of one Heterodera population were intercepted from seedlings carried by passenger at Shanghai port.Based on the morphological characters and molecular identification,the population was identified as Heterodera fici Kirjanova,1954.The morphological characters were mainly as follows:body length 409.9-454.4 μm;labial region with three annuli;stylet well developed,basal knob rounded,directed slightly anteriorly,22.6-24.0 μm long;lateral field with four incisures;tail long,tapering,terminus rounded;tail hyaline length 24.7-30.5 μm,about half of the tail length.The analysis of ITS region sequence showed 99.48％-99.90％ sequence identity with H.fici in GenBank.The phylogenetic tree based on ITS sequences revealed that the sample clustered with H.fici in GenBank.This is the first report of H.fici intercepted in China. |
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| Keywords: | Heteroderae fici morphological characters phylogeny identification |
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