| 李离体茎尖的超低温保存 |
| |
| 引用本文： | 赵艳华,吴雅琴,程和禾,李春敏,陈晓玲,卢新雄.李离体茎尖的超低温保存[J].园艺学报,2008,35(3):423-426. |
| |
| 作者姓名： | 赵艳华 吴雅琴 程和禾 李春敏 陈晓玲 卢新雄 |
| |
| 作者单位： | （河北省农林科学院昌黎果树研究所,河北昌黎066600；中国农业科学院作物科学研究所, 北京100081） |
| |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金 |
| |
| 摘 要： | 以简单玻璃化法为基本方法，研究了影响李离体茎尖超低温保存的因子—继代培养时间、低温驯化时间、PVS3处理时间以及化冻后茎尖存活检测；建立了较为简单的李离体茎尖超低温保存技术程序：即选择继代培养120 d的试管材料，经过5 ℃低温驯化21 d，0.7 mol·L-1蔗糖预培养1 d，PVS3处理100 min，浸入液氮。化冻后茎尖存活率可达60 %以上。
|
| 关 键 词： | 李 离体茎尖 超低温保存 |
| 收稿时间： | 2007-11-18 |
Cryopreservation of Shoot Tips from Prunus |
| |
| ZHAO Yan-hua,WU Ya-qin,CHENG He-he,LI Chun-min,CHEN Xiao-loing,LU Xin-xiong.Cryopreservation of Shoot Tips from Prunus[J].Acta Horticulturae Sinica,2008,35(3):423-426. |
| |
| Authors: | ZHAO Yan-hua WU Ya-qin CHENG He-he LI Chun-min CHEN Xiao-loing LU Xin-xiong |
| |
| Institution: | 1.Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital, Guangzhou Medical College, Guangzhou 510120, China;2.Key Laboratory for Functional Proteomics of Guangdong Province, Southern Medical University, Guangzhou 510515, China. E-mail:xufeili@vip.163.com |
| |
| Abstract: | The current paper studied factors that effect the cryopreservation of Prunus salicina Lindl in-vitro shoot tips. By using simple vitrification technique,factors like, culture time, PVS3 treatment time and cold hardening were all tested ,the rapid examination method of shoot tips survival was studied,and a sample procedure was established at last.When shoot tips were excised from healthy in vitro plants of prunus cultivars,that have been subcultured for 120d and followed by 5 ℃ cold hardening for 21d ,and precultured with 0.7 mol.L-1 sucrose for 2d, treated with PVS3 for 100 min before direct plunging into liquid nitrogen,the survival after cryopreservation was higher than 60.0%. |
| |
| Keywords: | Prunus Prunus shoot tips cryopreservation |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
