| 苜蓿多糖对新城疫弱毒苗免疫效果的影响 |
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| 引用本文： | 张月,房琳琳,赵亚男,于洋,李敬双.苜蓿多糖对新城疫弱毒苗免疫效果的影响[J].现代畜牧兽医,2014(7):30-34. |
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| 作者姓名： | 张月 房琳琳 赵亚男 于洋 李敬双 |
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| 作者单位： | 辽宁医学院,辽宁锦州121001 |
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| 基金项目： | 辽宁省大学生创新创业训练计划项目“苜蓿多糖对新城疫疫苗免疫雏鸡外周血T细胞转化和血清抗体效价的影响”（201310160024）;辽宁省自然科学基金“苜蓿多糖对小鼠树突状细胞和巨噬细胞功能的调节作用”（201202138） |
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| 摘 要： | 为了研究苜蓿多糖对新城疫弱毒苗免疫鸡所产生的免疫调节作用,本研究选择1日龄海兰雏鸡120羽,随机分为4组,每组30羽,7日龄每羽用鸡新城疫活疫苗（La Sota株）免疫,28日龄以相同剂量进行加强免疫。其中3个试验组从7日龄开始分别肌肉注射低、中、高剂量（10、25、50μg/mL）的苜蓿多糖,对照组注射生理盐水,1次/d,连用7 d。于14、21、28、35、42、49、56日龄,通过静脉采血,用MTT法检测外周血淋巴细胞吸光度值（OD值）;同时用微量法检测血清中新城疫病毒血凝抑制（HI）抗体效价。结果显示,苜蓿多糖能够显著提高外周血淋巴细胞OD值,中剂量组（25μg/mL）淋巴细胞OD值最大;苜蓿多糖能够显著提高血清中新城疫病毒HI抗体效价,中剂量组免疫后期抗体存在时间长,抗体高峰期持续时间也长。本试验表明合适剂量苜蓿多糖对新城疫弱毒苗的免疫效果有明显的增强作用。
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| 关 键 词： | 苜蓿多糖 免疫 效果 |
Influence of the alfalfa polysaccharide on vaccine immunization effect of Newcastle disease |
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| Zhang Yue;Fang Linlin;Zhao Yanan;Yu Yang;Li Jingshuang.Influence of the alfalfa polysaccharide on vaccine immunization effect of Newcastle disease[J].Modern JOurnal of Animal Husbandry and Veterinary Medicine,2014(7):30-34. |
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| Authors: | Zhang Yue;Fang Linlin;Zhao Yanan;Yu Yang;Li Jingshuang |
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| Institution: | Zhang Yue;Fang Linlin;Zhao Yanan;Yu Yang;Li Jingshuang;(Liaoning Medical University; Liaoning Jinzhou 121001) |
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| Abstract: | In order to observe the immunoregulatory effects of alfalfa polysaccharide on Newcas-tle disease vaccine immunization, 120 1-day-age chickens were selected and randomly divided into four groups, 30 chickens per group. La Sota strain immunization and booster were performed at day-7and day-28. From day-7, three test group chickens were inoculated diffraction dose （10, 25, 50 μg/mL） of alfalfa polysaccharide muscle injection each day, and control chickens were inject-ed physiological saline water synchronously.At the day-14, 21, 28, 35, 42, 49 and 56, all chick-ens were blooded and the peripheral blood lymphocyte absorbance value （OD value） was detected by MTT method. The serum antibody titers of NDV were determined by hemagglutination inhibition （HI） method. As a results, alfalfa polysaccharide can improve the OD value of peripheral blood lympho-cytes transformation and the OD values most significantly with dose of 25 μg/mL, meanwhile, al-falfa polysaccharide can significantly increase the HI antibody titer in serum and maintain late high immune antibody level long time, antibody peak period showed longer than control groups. The experiment showed that appropriate doses of alfalfa polysaccharides obviously enhanced the immune effect of Newcastle disease vaccine immunization effect. |
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| Keywords: | Alfalfa polysaccharides Immunization Effect |
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