Molecular Phylogenetic Analysis of Ribosomal DNA Internal Transcribed Spacer Regions and Comparison of Fertility in <Emphasis Type="Italic">Phomopsis </Emphasis>Isolates from Fruit Trees |
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| Authors: | Satoko KANEMATSU Nobuhiro MINAKA Takao KOBAYASHI Akira KUDO Yoshihiro OHTSU |
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| Institution: | (1) National Institute of Fruit Tree Science, Tsukuba 305–8605, Japan, JP;(2) Apple Research Center, National Institute of Fruit Tree Science, Morioka 020–0123, Japan, JP;(3) National Institute of Agro-Environmental Sciences, Tsukuba 305–8604, Japan, JP;(4) Department of International Agricultural Development, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156–8502, Japan, JP |
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| Abstract: | Sequences of the ribosomal DNA (rDNA) internal transcribed spacer (ITS) regions were examined to infer a molecular phylogeny
of small-spored Phomopsis isolates, designated W-type (mainly white colony, weakly virulent, bearing both alpha and beta conidia at 25°C on PDA) and
G-type (mainly gray colony, highly virulent, bearing only alpha conidia at 25°C on PDA), and P. amygdali from fruit trees. Phomopsis G-type and P. amygdali were a monophyletic group distinct from the W-type. The W-type isolates were divided into two monophyletic groups. Diaporthe citri, D. tanakae, P. asparagi, P. viticola, P. vitimegaspora and D. nomurai, which are morphologically distinguishable from W- and G-types, differed from the W- and G-types in molecular phylogenetic
analyses. PCR-RFLP analysis of rDNA ITS regions was useful to distinguish each of the Phomopsis species and groups using three restriction enzymes. In mating tests, W-type isolates from fruit trees were heterothallic and
inter-fertile even between isolates belonging to the different monophyletic groups. Isolates of the G-type and P. amygdali collected in Japan were cross-fertile. Some isolates from Lunaria annua, Ulmus glabra and Juglans regia belonged to one of the two monophyletic groups of the W-type and were cross-fertile with W-type isolates from Rosaceous fruit
trees.
Received 27 September 1999/ Accepted in revised form 27 January 2000 |
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| Keywords: | : Diaporthe eres Diaporthe medusaea Phomopsis amygdali rDNA ITS regions mating taxonomy |
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