Characterization of isozyme variation in walnut(Juglans regia L.) |
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| Authors: | A Solar J Smole F ?tampar M Vir??ek-Marn |
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| Institution: | (1) Biotechnical Faculty, Agronomy Department, University of Ljubljana, 61000 Ljubljana, Slovenia |
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| Abstract: | Summary Four leaf enzymes malate dehydrogenase (MDH), 6-phosphogluconate dehydrogenase (6PGD), peroxidase (PX) and aspartate aminotransferase
(AAT) of 17 walnut cultivars and two pollen enzymes malate dehydrogenase (MDH) and 6-phosphogluconate dehydrogenase (6PGD)
of 15 walnut cultivars were analysed using horizontal starch gel electrophoresis. Walnut cultivars of different origin exhibited
different numbers of electrophoretic bands and also different relative mobility. Different activity levels and phenotypic
groups were detected in studied enzyme systems. Pollen enzymes revealed higher variability than enzymes extracted from the
leaves. 15 walnut cultivars were classified into 10 malate dehydrogenase phenotypic groups and 14 6-phosphogluconate dehydrogenase
phenotypic groups based on pollen analyses. 17 cultivars were classified into 9 peroxidase phenotypic groups and 7 6-phosphogluconate
dehydrogenase phenotypic groups based on analyses of leaves. All of the 15 walnut cultivars could be identified and distinguished
with electrophoretic analyses of MDH and 6PGD from the pollen while only 10 cultivars were distinguishable with analyses of
6PGD and PRX from the leaves. No variability useful for cultivar identification was observed in MDH and AAT from the leaves. |
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| Keywords: | Cultivar identification isozyme variation walnut Juglans regia L |
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