Detection of a broad range of potato viruses in a single assay by mechanical inoculation of herbaceous test plants |
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| Authors: | J T J Verhoeven J W Roenhorst |
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| Institution: | Plant Protection Service, PO Box 9102, 6700 HC Wageningen (the Netherlands);e-mail: |
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| Abstract: | To optimize mechanical inoculation of test plants as part of the regulated post-entry quarantine testing of stolon- and tuber-forming Solanum spp. (including potato), nine test plant species have been screened for their ability to detect 22 potato-infecting viruses. These included all the common mechanically transmissible potato viruses and most of the viruses found in potato incidentally. The symptoms observed after mechanical inoculation are shown for each combination of test plant species and virus. Under given conditions, Nicotiana occidentalis -P1 and Nicotiana hesperis -67A were found to detect reliably 20 and 18 out of 22 viruses respectively. These and former results on the seed-transmissible viruses and Andean potato mottle virus demonstrate that these Nicotiana species are very suitable for post-entry quarantine testing. Addition of either Chenopodium amaranticolor or Chenopodium quinoa to these two species may slightly extend the range of viruses. Therefore, using C. amaranticolor / C. quinoa , N. hesperis -67A and N. occidentalis -P1 for the biological screening of imported Solanum spp. will improve both the efficiency and the quality of post-entry quarantine testing. |
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